Ganesh Arathi, Veronica Aruna Kumari, Ashok Rupa, Varadan Preethi, Deivanayagam Kandaswamy
Conservative Dentistry and Endodontics, Sri Ramachandra Institute of Higher Education and Research, Chennai, IND.
Conservative Dentistry and Endodontics, Madha Dental College, Chennai, IND.
Cureus. 2019 May 21;11(5):e4711. doi: 10.7759/cureus.4711.
Microorganisms have been known to cause pain and infection in the tooth. Fusobacterium nucleatum was always found predominantly in failed root canal treatments.
The aim of the present study was to quantify Fusobacterium nucleatum at the inner and peripheral half of coronal, middle and apical region of the root by using real-time polymerase chain reaction (qPCR).
Extracted maxillary incisors were taken. After shaping and cleaning, the root canals were inoculated with Fusobacterium nucleatum. Samples were taken from both the inner and peripheral halves of dentin. The inoculated teeth were maintained in anaerobic jars for two weeks, and the bacterial isolates were changed every third day. The quantification was done using qPCR.
The cycle threshold (Ct) value in all groups showed the presence of Fusobacterium nucleatum.
Fusobacterium nucleatum penetrates to the entire thickness of dentin in the middle and apical region. The coaggregation with other microorganisms could be responsible for the symptomatic endodontic patients.
已知微生物会导致牙齿疼痛和感染。具核梭杆菌在根管治疗失败病例中总是大量存在。
本研究的目的是通过实时聚合酶链反应（qPCR）对具核梭杆菌在牙根冠部、中部和根尖部内侧及外周半层进行定量分析。
取拔除的上颌切牙。根管预备和清理后，向根管内接种具核梭杆菌。从牙本质内侧和外周半层取样。接种后的牙齿置于厌氧罐中两周，每三天更换一次细菌分离物。使用qPCR进行定量分析。
所有组的循环阈值（Ct）值表明存在具核梭杆菌。
具核梭杆菌可穿透牙根中部和根尖部牙本质的全层。与其他微生物的共聚可能是导致症状性牙髓病患者的原因。
