Hu Chao, Liu Yu-Xi, Zhang Shi-Pang, Wang Ya-Qi, Gao Ping, Li Yu-Ting, Yang Xue-Qing
College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China.
Key Laboratory of Economical and Applied Entomology of Liaoning Province, Shenyang 110866, Liaoning, China.
J Agric Food Chem. 2023 Apr 5;71(13):5230-5239. doi: 10.1021/acs.jafc.3c00002. Epub 2023 Mar 21.
Aryl hydrocarbon receptor (AhR) enhances insect resistance to insecticides by regulating the detoxification network. Our previous studies have confirmed that overexpressions of cytochrome P450 monooxygenases (P450s) and glutathione -transferases (GSTs) are involved in -cyhalothrin resistance in . Here, we report that regulates the expression of GST and P450 genes, thus conferring resistance. Expression patterns indicated that the expression of was highly induced by -cyhalothrin exposure and upregulated in a -cyhalothrin-resistant population. RNA interference (RNAi) of decreases the expression of key resistance-related genes (, , and ) and the activity of the GST enzyme, reducing the tolerance to -cyhalothrin. Furthermore, β-naphthoflavone, a novel agonist of AhR, was first proven to be effective in increasing expression and larval tolerance to -cyhalothrin. These results demonstrate that regulates the expression of key detoxifying genes and GST activity, resulting in the development of resistance to -cyhalothrin in .
芳基烃受体（AhR）通过调节解毒网络增强昆虫对杀虫剂的抗性。我们之前的研究证实，细胞色素P450单加氧酶（P450s）和谷胱甘肽-S-转移酶（GSTs）的过表达与[昆虫名称]对氯氟氰菊酯的抗性有关。在此，我们报道[AhR]调节GST和P450基因的表达，从而赋予抗性。表达模式表明，[AhR]的表达在接触氯氟氰菊酯后被高度诱导，并且在对氯氟氰菊酯具有抗性的种群中上调。对[AhR]进行RNA干扰（RNAi）会降低关键抗性相关基因（[具体基因名称1]、[具体基因名称2]和[具体基因名称3]）的表达以及GST酶的活性，降低对氯氟氰菊酯的耐受性。此外，β-萘黄酮，一种新型的AhR激动剂，首次被证明可有效增加[AhR]的表达以及幼虫对氯氟氰菊酯的耐受性。这些结果表明，[AhR]调节关键解毒基因的表达和GST活性，导致[昆虫名称]对氯氟氰菊酯产生抗性。
