Transcription Factor AhR Regulates Glutathione -Transferases Conferring Resistance to -Cyhalothrin in .

Aryl hydrocarbon receptor (AhR) enhances insect resistance to insecticides by regulating the detoxification network. Our previous studies have confirmed that overexpressions of cytochrome P450 monooxygenases (P450s) and glutathione -transferases (GSTs) are involved in -cyhalothrin resistance in . Here, we report that regulates the expression of GST and P450 genes, thus conferring resistance. Expression patterns indicated that the expression of was highly induced by -cyhalothrin exposure and upregulated in a -cyhalothrin-resistant population. RNA interference (RNAi) of decreases the expression of key resistance-related genes (, , and ) and the activity of the GST enzyme, reducing the tolerance to -cyhalothrin. Furthermore, β-naphthoflavone, a novel agonist of AhR, was first proven to be effective in increasing expression and larval tolerance to -cyhalothrin. These results demonstrate that regulates the expression of key detoxifying genes and GST activity, resulting in the development of resistance to -cyhalothrin in .