Orthopedics and Sports Medicine, Hospital Médica Sur, Mexico, Mexico.
Cartilage Research Unit at University of Gothenburg, Orthopedic Surgeon at Region Halland Orthopaedics at the Kungsbacka Hospital Kungsbacka, Gothenburg, Sweden.
Cell Tissue Bank. 2023 Dec;24(4):725-735. doi: 10.1007/s10561-023-10074-4. Epub 2023 Mar 22.
Autologous chondrocyte implantation has shown optimal long-term outcomes in the treatment of cartilage lesions. The challenge for a single-stage approach lies in obtaining sufficient number of cells with high viability. The answer could lie in supplementing or replacing them with allogenic chondrocytes coming from cadaveric donors. In the present work, we aimed to compare the number of viable cells isolated from cartilage of live and cadaveric donors and to determine the suitable characteristics of the best donors. A total of 65 samples from donors aged from 17 to 55 years, either women or men, were enrolled in this study (33 living vs. 32 cadaveric). The mean time of hours from death to processing samples in cadaveric donors was higher compared to live donors (64.3 ± 17.7 vs. 4.6±6.4). The number of isolated chondrocytes per gram of cartilage was higher in cadaveric donors (5.389 × 10 compared to 3.067 × 10 in living donors), whereas the average of cell viability was comparable in both groups (84.16% cadaveric, 87.8% alive). It is possible to obtain viable chondrocytes from cartilage harvested from cadaveric donors, reaching a similar cell number and viability to that obtained from the cartilage of living donors.
自体软骨细胞移植在治疗软骨损伤方面显示出了最佳的长期效果。对于单阶段方法的挑战在于获得具有高活力的足够数量的细胞。答案可能在于用来自尸体供体的同种异体软骨细胞补充或替代它们。在本工作中，我们旨在比较从活体和尸体供体的软骨中分离出的活细胞数量，并确定最佳供体的合适特征。共纳入了 65 名年龄在 17 至 55 岁之间的供体（33 名活体供体与 32 名尸体供体）的样本。与活体供体相比，尸体供体的从死亡到处理样本的小时数平均更高（64.3 ± 17.7 对 4.6 ± 6.4）。每克软骨分离的软骨细胞数量在尸体供体中更高（5.389×10 对活体供体中的 3.067×10），而两组的细胞活力平均值相当（84.16%的尸体，87.8%的活体）。从尸体供体采集的软骨中可以获得具有活力的软骨细胞，达到与从活体供体软骨中获得的类似细胞数量和活力。
