School of Dentistry, Chung Shan Medical University, Taichung, Taiwan.
Department of Dentistry, Chung Shan Medical University Hospital, Taichung, Taiwan.
J Cell Mol Med. 2023 Apr;27(8):1144-1152. doi: 10.1111/jcmm.17728. Epub 2023 Mar 22.
A disintegrin and metalloproteinase domain-containing protein 10 (ADAM-10) involves in the tumour progression, but the impacts of single-nucleotide polymorphism (SNP) of ADAM-10 on oral squamous cell carcinoma (OSCC) remain unclear. The aim of this study was to investigate the influence of SNP of ADAM-10 on the clinical features of OSCC in male Taiwanese. Five loci of ADAM-10 SNPs including rs653765 (C/T), rs2305421 (A/G), rs514049 (A/C), rs383902 (T/C) and rs2054096 (A/T) were genotyped by TaqMan allelic discrimination in 1138 OSCC patients and 1199 non-OSCC individuals. The ADAM-10 SNP rs2305421 GG (AOR: 1.399, 95% CI: 1.045-1.874, p = 0.024) and G allele (AOR: 1.170, 95% CI: 1.012-1.351, p = 0.034) illustrated a significantly higher genotypic frequencies in the OSCC group compared to the distribution of the ADAM-10 SNP rs2305421 AA wild type. In the subgroup analysis, the ADAM-10 SNP rs383902 TC+CC was significantly correlated to tumour size larger than T2 in betel quid chewer (AOR: 1.375, 95% CI: 1.010-1.872, p = 0.043), while the ADAM-10 SNP rs653765 CT+TT was significantly associated with tumour size larger than T2 in cigarette smoker (AOR: 1.346, 95% CI: 1.023-1.772, p = 0.034). The results from The Cancer Genome Atlas revealed highest ADAM-10 mRNA level in T2 stage of current smokers with head and neck squamous cell carcinoma (HNSCC). In conclusions, the ADAM-10 SNP rs2305421 G allele is associated with the presence of OSCC, and the ADAM-10 SNP rs383902 TC+CC and ADAM-10 SNP rs653765 CT+TT correlates to large tumour size in specific conditions.
解整合素金属蛋白酶 10（ADAM-10）参与肿瘤的进展，但 ADAM-10 单核苷酸多态性（SNP）对口腔鳞状细胞癌（OSCC）的影响尚不清楚。本研究旨在探讨 ADAM-10 SNP 对台湾男性 OSCC 临床特征的影响。通过 TaqMan 等位基因鉴别法对 1138 例 OSCC 患者和 1199 例非 OSCC 个体的 ADAM-10 5 个SNP （rs653765 [C/T]、rs2305421 [A/G]、rs514049 [A/C]、rs383902 [T/C]和 rs2054096 [A/T]）进行基因分型。ADAM-10 SNP rs2305421 GG（OR：1.399，95%CI：1.045-1.874，p=0.024）和 G 等位基因（OR：1.170，95%CI：1.012-1.351，p=0.034）在 OSCC 组中的基因型频率明显高于 ADAM-10 SNP rs2305421 AA 野生型的分布。在亚组分析中，ADAM-10 SNP rs383902 TC+CC 与咀嚼槟榔者 T2 以上肿瘤大小显著相关（OR：1.375，95%CI：1.010-1.872，p=0.043），而 ADAM-10 SNP rs653765 CT+TT 与吸烟者 T2 以上肿瘤大小显著相关（OR：1.346，95%CI：1.023-1.772，p=0.034）。来自癌症基因组图谱的数据显示，当前吸烟者头颈部鳞状细胞癌（HNSCC）T2 期 ADAM-10 mRNA 水平最高。总之，ADAM-10 SNP rs2305421 G 等位基因与 OSCC 的存在相关，而 ADAM-10 SNP rs383902 TC+CC 和 ADAM-10 SNP rs653765 CT+TT 与特定条件下的大肿瘤大小相关。
