Bai Qiulu, Zhang Xiaowen, Wang Wei, Zhang Huiyun, He Shaoheng
Translational Medicine Research Center, School of Basic Medicine, Shenyang Medical College, Shenyang 110000, China.
Research Center for Allergy and Clinical Immunity, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2023 Mar;39(3):193-198.
Objective To identify the expression of Toll-like receptor 2 (TLR2) on peripheral blood monocytes and B cells of patients with allergic rhinitis (AR), allergic rhinitis combined with allergic asthma (ARA) before and after allergen challenge. Methods The peripheral venous blood from patients with AR and ARA were recruited and stimulated with Artemisia sieversiana wild allergen extract (ASWE), house dust mite allergen extract (HDME), and Platanus pollen allergen extract (PPE). Flow cytometry was then used to detect the expression of TLR2 on peripheral monocytes and B cells. Results Compared with healthy control (HC) group, the percentage of TLR2 monocytes and decreased mean fluorescence intensity (MFI) of TLR2 on monocytes in AR and ARA patients decreased. After being challenged with the above mentioned three allergens, the portion of TLR2 monocytes in HC group and MFI of TLR2 on monocytes in AR patients also decreased. Meanwhile, MFI of TLR2 on B cells also showed a decrease after challenged with ASWE and HDME. Conclusion The expression of TLR2 on monocytes and B cells decreases in AR and ARA patients.
目的 鉴定变应性鼻炎（AR）、变应性鼻炎合并变应性哮喘（ARA）患者在变应原激发前后外周血单核细胞和B细胞上Toll样受体2（TLR2）的表达。方法 招募AR和ARA患者的外周静脉血，用西伯利亚艾蒿变应原提取物（ASWE）、屋尘螨变应原提取物（HDME）和悬铃木花粉变应原提取物（PPE）进行刺激。然后采用流式细胞术检测外周单核细胞和B细胞上TLR2的表达。结果 与健康对照（HC）组相比，AR和ARA患者中TLR2单核细胞的百分比以及单核细胞上TLR2的平均荧光强度（MFI）降低。用上述三种变应原激发后，HC组中TLR2单核细胞的比例以及AR患者单核细胞上TLR2的MFI也降低。同时，用ASWE和HDME激发后，B细胞上TLR2的MFI也降低。结论 AR和ARA患者单核细胞和B细胞上TLR2的表达降低。
