Electrical Engineering Division, Department of Engineering, University of Cambridge, Cambridge, UK.
Department of Clinical Neurosciences, University of Cambridge, Cambridge, UK.
Sci Adv. 2023 Mar 22;9(12):eadd8162. doi: 10.1126/sciadv.add8162.
The development of neural interfaces with superior biocompatibility and improved tissue integration is vital for treating and restoring neurological functions in the nervous system. A critical factor is to increase the resolution for mapping neuronal inputs onto implants. For this purpose, we have developed a new category of neural interface comprising induced pluripotent stem cell (iPSC)-derived myocytes as biological targets for peripheral nerve inputs that are grafted onto a flexible electrode arrays. We show long-term survival and functional integration of a biohybrid device carrying human iPSC-derived cells with the forearm nerve bundle of freely moving rats, following 4 weeks of implantation. By improving the tissue-electronics interface with an intermediate cell layer, we have demonstrated enhanced resolution and electrical recording in vivo as a first step toward restorative therapies using regenerative bioelectronics.
为了治疗和恢复神经系统的神经功能，开发具有优越生物相容性和改善组织整合性的神经接口至关重要。一个关键因素是提高将神经元输入映射到植入物上的分辨率。为此，我们开发了一种新型的神经接口，该接口由诱导多能干细胞（iPSC）衍生的心肌细胞组成，作为移植到柔性电极阵列上的周围神经输入的生物靶标。我们展示了携带人 iPSC 衍生细胞的生物杂交设备在植入 4 周后与自由移动大鼠的前臂神经束的长期存活和功能整合。通过用中间细胞层改善组织-电子界面，我们已经证明了体内分辨率和电记录的提高，这是使用再生生物电子学进行修复治疗的第一步。
