Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21102 Novi Sad, Serbia.
Faculty of Chemistry and Technology, University of Split, Ruđera Boškovića 35, 21000 Split, Croatia.
Molecules. 2022 Jun 17;27(12):3883. doi: 10.3390/molecules27123883.
High-pressure pre-treatment followed by supercritical carbon dioxide (ScCO) extraction (300 bar, 40 °C) was applied for the attainment of the lipophilic fraction of microalga . The chemical profile of supercritical extracts of was analyzed by ultra-high-performance liquid chromatography-high-resolution mass spectrometry with electrospray ionization (UHPLC-ESI-HRMS). Moreover, the impact of ScCO on the microbiological and metal profile of the biomass was monitored. The application of the pre-treatment increased the extraction yield approximately three-fold compared to the control. In the obtained extracts (control and pre-treated extracts), the identified components belonged to triacylglyceroles, fatty acid derivatives, diacylglycerophosphocholines and diacylglycerophosphoserines, pigments, terpenes, and steroids. Triacylglycerols (65%) were the most dominant group of compounds in the control extract. The pre-treatment decreased the percentage of triacylglycerols to 2%, while the abundance of fatty acid derivatives was significantly increased (82%). In addition, the pre-treatment led to an increase in the percentages of carotenoids, terpenoids, and steroids. Furthermore, it was determined that ScCO extraction reduced the number of microorganisms in the biomass. Considering its microbiological and metal profiles, the biomass after ScCO can potentially be used as a safe and important source of organic compounds.
采用高压预处理结合超临界二氧化碳（ScCO）萃取（300 巴，40°C）的方法从微藻中提取亲脂性部分。采用超高效液相色谱-高分辨质谱联用技术（UHPLC-ESI-HRMS）分析 的超临界提取物的化学特征。此外，还监测了 ScCO 对生物质微生物和金属特征的影响。与对照组相比，预处理将萃取产率提高了约三倍。在获得的提取物（对照组和预处理提取物）中，鉴定出的成分属于三酰基甘油、脂肪酸衍生物、二酰基甘油磷酸胆碱和二酰基甘油磷酸丝氨酸、色素、萜类化合物和甾体化合物。三酰基甘油（65%）是对照组提取物中最主要的化合物组。预处理将三酰基甘油的比例降低至 2%，而脂肪酸衍生物的丰度显著增加（82%）。此外，预处理还导致类胡萝卜素、萜类化合物和甾体化合物的比例增加。此外，还确定 ScCO 萃取降低了生物质中微生物的数量。考虑到其微生物和金属特征，经过 ScCO 处理后的生物质可能可作为安全且重要的有机化合物来源。
