Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, PR China.
Department of Materials Science and Engineering, College of Chemistry and Materials Science, Jinan University, Guangzhou 511443, PR China.
Int J Biol Macromol. 2023 May 15;237:124148. doi: 10.1016/j.ijbiomac.2023.124148. Epub 2023 Mar 22.
Uncontrolled massive hemorrhage is a crucial cause of death, and developing efficient hemostatic materials are of great medical importance. Herein, we prepared a halloysite-chitosan-collagen composite sponge by directional freeze-drying method and coated the sponge with hydrophobic polydimethylsiloxane coating for rapid and effective hemostasis. The aligned channel structure of the sponge with a pore size of ~30 μm was beneficial for the transport of blood. Morphology and spectrum results suggested that chitosan and collagen are capable of adsorbing on the outer surface of HNTs due to the hydrogen bonding and electrostatic attractions. The directional freeze-dried sponge absorbed the majority of the blood within 10 s, and that process essentially was completed in 30 s, which are faster than its non-directional counterpart. The composite sponges exhibited high antibacterial properties towards E. coli and S. aureus, and they are non-cytotoxic towards mouse fibroblasts and have high hemocompatibility. The hemostatic dressing avoided unnecessary blood loss because of excessive blood absorption. In vivo experiments of rats also confirmed the ability of the asymmetric sponges to rapidly clot and reduce reducing blood loss. This work developed a high-performance and hemostatic dressing by material design and processing technique, which shows a promising application in wound healing.
失控性大出血是导致死亡的一个重要原因，因此开发高效的止血材料具有重要的医学意义。在此，我们通过定向冷冻干燥法制备了海泡石-壳聚糖-胶原复合海绵，并对其进行了疏水性聚二甲基硅氧烷涂层处理，以实现快速有效的止血。海绵具有约 30μm 的孔径的定向排列通道结构有利于血液的传输。形貌和光谱结果表明，壳聚糖和胶原由于氢键和静电吸引作用能够吸附在 HNTs 的外表面上。定向冷冻干燥海绵在 10s 内吸收了大部分血液，这一过程在 30s 内基本完成，比非定向海绵更快。该复合海绵对大肠杆菌和金黄色葡萄球菌具有较高的抗菌性能，对小鼠成纤维细胞无细胞毒性，具有较高的血液相容性。止血敷料避免了因过度吸收血液而导致的不必要的失血。大鼠体内实验也证实了不对称海绵能够快速止血和减少失血的能力。这项工作通过材料设计和加工技术开发了一种高性能的止血敷料，有望在伤口愈合方面得到应用。
