Saharkhiz Shaghayegh, Zarepour Atefeh, Zarrabi Ali
Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.
Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Istinye University, Istanbul 34396, Turkey.
Int J Pharm. 2023 Apr 25;637:122845. doi: 10.1016/j.ijpharm.2023.122845. Epub 2023 Mar 22.
As one of the newest generations of nanoplatforms, smart nanotheranostics have attracted signifivant attentions for medical applications, especially in oncology and cancer treatment. Indeed, their capability to provide treatment and diagnosis simultaneously leads to reduce time and side effects along with improving the performance. This study aims to introduce a novel smart nano-platform composed of doxorubicin-loaded pH-responsive stealth niosomes containing CdSe/ZnS Quantum dots as an imaging agent. Drug loaded nano-platform was fabricated via thin-film hydration method and then evaluated using different physicochemical tests. The entrapment efficiency and release profile of doxorubicin were assessed at three different pH (4, 6.5, and 7.4). Biological features and imaging ability of the nanoparticles were also evaluated by MTT assay, apoptosis assay, and fluorescence microscopy. Results showed that the fabricated nanoparticles were round-shaped, with a mean size of about 100 ± 10 nm, -2 mV surface charge, and about 87% entrapment efficiency. The drug release profile presented a pH-responsive behavior (80, 60, and 40% drug release in pH 4, 6.5, and 7.4, respectively). The bio-activity assessments showed nearly 55% cytotoxicity effects via inducing cell apoptosis. Besides, the uptake of samples by the cells was confirmed through fluorescence imaging. Based on the results, this new nanoformulation could be considered as a candidate for future cancer theranostic applications.
作为最新一代的纳米平台之一，智能纳米诊疗系统在医学应用领域，尤其是肿瘤学和癌症治疗方面，已引起了广泛关注。事实上，它们同时提供治疗和诊断的能力，有助于减少时间和副作用，同时提高治疗效果。本研究旨在介绍一种新型智能纳米平台，该平台由负载阿霉素的pH响应型隐形脂质体组成，并含有CdSe/ZnS量子点作为成像剂。通过薄膜水化法制备了载药纳米平台，然后使用不同的物理化学测试方法对其进行评估。在三种不同的pH值（4、6.5和7.4）下评估了阿霉素的包封率和释放曲线。还通过MTT法、凋亡检测和荧光显微镜对纳米颗粒的生物学特性和成像能力进行了评估。结果表明，制备的纳米颗粒呈圆形，平均粒径约为100±10nm，表面电荷为-2mV，包封率约为87%。药物释放曲线呈现出pH响应行为（在pH 4、6.5和7.4时，药物释放率分别为80%、60%和40%）。生物活性评估显示，通过诱导细胞凋亡，具有近55%的细胞毒性作用。此外，通过荧光成像证实了细胞对样品的摄取。基于这些结果，这种新的纳米制剂可被视为未来癌症诊疗应用的候选者。
