RNA-seq 和 microRNA 关联分析探索 DEHs 中 DHAV-1 感染的致病机制。

RNA-seq and microRNA association analysis to explore the pathogenic mechanism of DHAV-1 infection with DEHs.

机构信息

MOE Joint International Research Laboratory of Animal Health and Food Safety and Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, 210095, Nanjing, People's Republic of China.

Beijing Key Laboratory of Traditional Chinese Veterinary Medicine, Beijing University of Agriculture, Beijing, 102206, People's Republic of China.

出版信息

Funct Integr Genomics. 2023 Mar 23;23(2):99. doi: 10.1007/s10142-023-01022-2.

DOI:10.1007/s10142-023-01022-2

PMID:36959488

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10035973/

Abstract

Duck hepatitis A virus 1 (DHAV-1) is one of the main contagious pathogens that causes rapid death of ducklings. To illuminate the potential of DHAV-1-infected underlying mechanisms, we analyzed the mRNA and microRNA (miRNA) expression profiles of duck embryonic hepatocytes (DEHs) in response to DHAV-1. We found 3410 differentially expressed genes (DEGs) and 142 differentially expressed miRNAs (DEMs) at 36 h after DHAV-1 infection. Additionally, DEGs and the target genes of miRNA expression were analyzed and enriched utilizing GO and KEGG, which may be crucial for immune responses, viral resistance, and mitophagy. For instance, the dysregulation of DDX58, DHX58, IRF7, IFIH1, STING1, TRAF3, CALCOCO2, OPTN, PINK1, and MFN2 in DHAV-1-infected DEHs was verified by RT-qPCR. Then, the association analysis of mRNAs and miRNAs was constructed utilizing the protein-protein interaction (PPI) networks, and the expressions of main miRNAs were confirmed, including miR-132c-3p, miR-6542-3p, and novel-mir163. These findings reveal a synthetic characterization of the mRNA and miRNA in DHAV-1-infected DEHs and advance the understanding of molecular mechanism in DHAV-1 infection, which may provide a hint for the interactions of virus and host.

摘要

鸭甲型肝炎病毒 1（DHAV-1）是引起雏鸭快速死亡的主要传染性病原体之一。为了阐明 DHAV-1 感染的潜在机制，我们分析了感染 DHAV-1 后鸭胚肝细胞（DEH）的 mRNA 和 microRNA（miRNA）表达谱。我们发现，DHAV-1 感染后 36 小时有 3410 个差异表达基因（DEGs）和 142 个差异表达 miRNA（DEM）。此外，利用 GO 和 KEGG 对 DEGs 和 miRNA 表达的靶基因进行了分析和富集，这可能对免疫反应、抗病毒和线粒体自噬至关重要。例如，通过 RT-qPCR 验证了 DHAV-1 感染的 DEH 中 DDX58、DHX58、IRF7、IFIH1、STING1、TRAF3、CALCOCO2、OPTN、PINK1 和 MFN2 的失调。然后，利用蛋白质-蛋白质相互作用（PPI）网络构建了 mRNA 和 miRNA 的关联分析，并验证了主要 miRNA 的表达，包括 miR-132c-3p、miR-6542-3p 和 novel-mir163。这些发现揭示了 DHAV-1 感染的 DEH 中 mRNA 和 miRNA 的综合特征，加深了对 DHAV-1 感染分子机制的理解，为病毒与宿主的相互作用提供了线索。