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局部用氟化物应用对患有严重龋齿的幼儿口腔微生物群的影响。

Effects of topical fluoride application on oral microbiota in young children with severe dental caries.

机构信息

Department of Preventive Dentistry, Stomatological Hospital of Chongqing Medical University, Chongqing, China.

Chongqing Key Laboratory of Oral Biomedical Engineering of Higher Education, Department of Preventive Dentistry, Chongqing, China.

出版信息

Front Cell Infect Microbiol. 2023 Mar 7;13:1104343. doi: 10.3389/fcimb.2023.1104343. eCollection 2023.

DOI:10.3389/fcimb.2023.1104343
PMID:36960045
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10028198/
Abstract

While the effect of fluoride on severe early childhood caries (S-ECC) is clear, knowledge of how it influences the oral microbiota and the consequential effects on oral health is limited. In this cohort study, we investigated the changes introduced in the oral ecosystem before and after using fluoride varnish in 54- to 66-month-old individuals (n=90: 18 children were sampled at 5 different time points). 16S rDNA was amplified from bacterial samples using polymerase chain reaction, and high-throughput sequencing was performed using Illumina MiSeq platforms. Many pronounced microbial changes were related to the effects of fluoride varnishing. The health-associated and were enriched in the saliva microbiome following treatment with fluoride varnishing. Co-occurrence network analysis of the dominant genera showed that different groups clearly showed different bacterial correlations. The PICRUSt algorithm was used to predict the function of the microbial communities from saliva samples. The results showed that starch and sucrose metabolism was greater after fluoride use. BugBase was used to determine phenotypes present in microbial community samples. The results showed that and (phylum ) was greater before fluoride use. We conclude that the changes in oral microbiology play a role in fluoride prevention of S-ECC.

摘要

虽然氟化物对严重婴幼儿龋（S-ECC）的影响是明确的，但关于它如何影响口腔微生物群以及对口腔健康的影响的知识是有限的。在这项队列研究中，我们调查了在 54-66 个月大的个体（n=90：18 名儿童在 5 个不同时间点采样）使用氟化物涂料前后引入的口腔生态系统的变化。使用聚合酶链反应从细菌样本中扩增 16S rDNA，并使用 Illumina MiSeq 平台进行高通量测序。许多明显的微生物变化与氟化物涂料的作用有关。在使用氟化物涂料处理后，口腔微生物组中与健康相关的 和 得到了富集。对优势属的共现网络分析表明，不同组清楚地显示出不同的细菌相关性。使用 PICRUSt 算法从唾液样本预测微生物群落的功能。结果表明，使用氟化物后淀粉和蔗糖代谢增加。使用 BugBase 确定微生物群落样本中存在的表型。结果表明，在使用氟化物之前， 和 （门）更多。我们得出结论，口腔微生物学的变化在氟化物预防 S-ECC 中发挥作用。

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本文引用的文献

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[Dynamic Microbial Shifts and Functional Analysis of Saliva Microbial Communities with Caries Children].[龋病儿童唾液微生物群落的动态变化及功能分析]
Sichuan Da Xue Xue Bao Yi Xue Ban. 2022 Mar;53(2):242-249. doi: 10.12182/20220360103.
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Fluoride Use in Caries Prevention in the Primary Care Setting.在初级保健环境中使用氟化物预防龋齿。
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Fluoride-Induced Alteration in the Diversity and Composition of Bacterial Microbiota in Mice Colon.氟化物诱导小鼠结肠细菌微生物群落多样性和组成的改变。
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The Oral Microbiome in the Elderly With Dental Caries and Health.老年人的口腔微生物组与龋齿和健康。
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