新种以及新种，从海洋沉积物中分离得到。
sp. nov. and sp. nov., isolated from marine sediment.
作者信息
Wang Ya-Wei, Zhang Jing, Wang Shu-Xin, Du Zong-Jun, Mu Da-Shuai
机构信息
Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, PR China.
出版信息
Int J Syst Evol Microbiol. 2023 Mar;73(3). doi: 10.1099/ijsem.0.005801.
Two Gram-stain-negative, strictly aerobic, chemoheterotrophic, short-rod-shaped and non-motile strains, forming yellow colonies and designated F47161 and F64183, were isolated from marine sediment of Xiaoshi Island, Wei Hai, PR China. Strain F47161 grew at 15-37 °C (optimally at 30 °C) and pH 6.0-9.0 (optimally at pH 7.5) and in the presence of 1-9 % (w/v) NaCl (optimally at 3 %). Strain F64183 grew at 10-37 °C (optimally at 30 °C) and pH 6.0-8.5 (optimally at pH 7.0) and in the presence of 1-8 % (w/v) NaCl (optimally at 3 %). The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that F47161 and F64183 were related to members of the genus . The strains shared 97.4 % 16S rRNA gene sequence similarity to each other. F47161 and F64183 shared highest 16S rRNA gene sequence similarity to JCM 19789, and the values were 97.5 % and 98.4 %, respectively. The predominant cellular fatty acids of both F64183 and F47161 were iso-C and iso-C 3-OH, but the predominant fatty acids of F47161 also included anteiso-C. The sole respiratory quinone of F47161 and F64183 was menaquinone 6 (MK-6), consistent with that observed for all related strains. Between F47161 and F64183, the average nucleotide identity (ANI) and digital DNA-DNA hybridisation (dDDH) values were 75.8 % and 20.5 %, respectively, and between the novel isolates (F47161 and F64183) and JCM 19789 they were 76.0 % and 94.2 % and 20.6 % and 57.1 %, respectively. The genomic DNA G+C contents of F47161 and F64183 was 37.3 % and 34.5 %, respectively. The polar lipid profiles of F47161 and F64183 contained phosphatidylethanolamine, two aminolipids, one glycolipid, one phosphoglycolipid and two unidentified polar lipids. Differential phenotypic and genotypic characteristics of the two strains indicated that the two strains should be classified as representing two novel species of the genus for which the names sp. nov. and sp. nov. are proposed. The type strains are F47161 (=MCCC 1H00509=KCTC 92017) and F64183 (=MCCC 1H00507=KCTC 92016), respectively.
从中国威海小石岛的海洋沉积物中分离出两株革兰氏阴性、严格需氧、化能异养、短杆状且无运动性的菌株，形成黄色菌落，分别命名为F47161和F64183。菌株F47161在15 - 37 °C（最适温度为30 °C）、pH 6.0 - 9.0（最适pH为7.5）以及1 - 9 %（w/v）NaCl（最适浓度为3 %）的条件下生长。菌株F64183在10 - 37 °C（最适温度为30 °C）、pH 6.0 - 8.5（最适pH为7.0）以及1 - 8 %（w/v）NaCl（最适浓度为3 %）的条件下生长。基于16S rRNA基因序列的系统发育分析结果表明，F47161和F64183与该属的成员相关。这两株菌株彼此间16S rRNA基因序列相似度为97.4 %。F47161和F64183与JCM 19789的16S rRNA基因序列相似度最高，分别为97.5 %和98.4 %。F64183和F47161的主要细胞脂肪酸均为异-C和异-C 3-OH，但F47161的主要脂肪酸还包括anteiso-C。F47161和F64183唯一的呼吸醌为甲基萘醌6（MK-6），这与所有相关菌株的情况一致。在F47161和F64183之间，平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值分别为75.8 %和20.5 %，在新分离菌株（F47161和F64183）与JCM 19789之间，ANI值分别为76.0 %和94.2 %，dDDH值分别为20.6 %和57.1 %。F47161和F64183的基因组DNA G+C含量分别为37.3 %和34.5 %。F47161和F64183的极性脂质谱包含磷脂酰乙醇胺、两种氨基脂质、一种糖脂、一种磷酸糖脂以及两种未鉴定的极性脂质。这两株菌株不同的表型和基因型特征表明，它们应被归类为该属的两个新物种，分别命名为sp. nov.和sp. nov.。模式菌株分别为F47161（=MCCC 1H00509=KCTC 92017）和F64183（=MCCC 1H00507=KCTC 92016）。
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