Liu Jun-Cheng, Ye Yu-Qi, Tan Xin-Yun, Du Zong-Jun, Ye Meng-Qi
SDU-ANU Joint Science College, Shandong University, Weihai 264209, China.
Marine College, Shandong University, Weihai 264209, China.
Microorganisms. 2023 Oct 9;11(10):2518. doi: 10.3390/microorganisms11102518.
A novel Gram-stain-negative, facultatively anaerobic, and non-motile bacterial strain, designated SDUM287046, was isolated from the coastal sediments of Jingzi Port of Weihai, China. Cells of strain SDUM287046 were rod-shaped with widths of 0.4-0.5 μm and lengths of 0.7-1.4 μm and could produce flexirubin-type pigments. Optimum growth of strain SDUM287046 occurred at 33-35 °C, pH 7.0, and with 2% (/) NaCl. Oxidase activity was negative, but catalase activity was positive. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain SDUM287046 was most closely related to D-24 (98.3%). The main cellular fatty acids were iso-C, anteiso-C, iso-C 3-OH, and summed feature 9 (comprised of iso-C ω9c and/or C 10-methyl). The sole respiratory quinone was MK-6. The polar lipids consisted of phosphatidylethanolamine (PE), one aminolipid (AL), three unidentified glycolipids (GL), and three unidentified lipids (L). The DNA G + C content was 39.3 mol%. According to the integrated results of phylogenetic, physiological, biochemical, and chemotaxonomic characteristics, we propose that strain SDUM287046 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SDUM287046 (=KCTC 92754 = MCCC 1H01418). Comparative genomic analysis showed that the 16 species shared 1453 core genes and differed mainly in amino acid metabolism, cofactor metabolism, and vitamin metabolism. Biogeographic distribution analysis indicated that the marine environments were the primary habitat of bacteria.
从中国威海靖子港的沿海沉积物中分离出一株新型革兰氏阴性、兼性厌氧且无运动性的细菌菌株，命名为SDUM287046。SDUM287046菌株的细胞呈杆状，宽度为0.4 - 0.5μm，长度为0.7 - 1.4μm，能够产生类胡萝卜素型色素。SDUM287046菌株在33 - 35°C、pH 7.0和2%（/）NaCl条件下生长最佳。氧化酶活性为阴性，但过氧化氢酶活性为阳性。基于16S rRNA基因序列的系统发育分析表明，SDUM287046菌株与D - 24关系最为密切（98.3%）。主要的细胞脂肪酸为异-C、anteiso-C、异-C 3-OH和总和特征9（由异-C ω9c和/或C 10-甲基组成）。唯一的呼吸醌为MK - 6。极性脂质由磷脂酰乙醇胺（PE）、一种氨基脂质（AL）、三种未鉴定的糖脂（GL）和三种未鉴定的脂质（L）组成。DNA G + C含量为39.3 mol%。根据系统发育、生理、生化和化学分类特征的综合结果，我们认为SDUM287046菌株代表了一个新的属种，为此提出新种名为sp. nov.。模式菌株为SDUM287046（=KCTC 92754 = MCCC 1H01418）。比较基因组分析表明，这16个物种共有1453个核心基因，主要在氨基酸代谢、辅因子代谢和维生素代谢方面存在差异。生物地理分布分析表明，海洋环境是该菌的主要栖息地。
