College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, Hubei Province, People's Republic of China.
Mikrochim Acta. 2023 Mar 24;190(4):155. doi: 10.1007/s00604-023-05735-w.
An electrochemiluminescence (ECL) sensor based on molecular imprinting polymer and SiO nanoparticles loaded Ru(bpy) and nitrogen-doped carbon quantum dots (NCQDs) is constructed for citrinin detection. The Ru(bpy) acts as ECL emitter, and the NCQDs cooperate with tri-n-propylamine (TPA) in solution as a coreactant to facilitate the luminescence. The citrinin imprinted poly(p-aminothiophenol) film is deposited on the surface of the luminophore by electrochemical method, which can immobilize the luminophore besides recognizing the target. The obtained ECL sensor exhibits high sensitivity, stability, and reproducibility. The change of ECL intensity and the logarithm of citrinin concentration display a good linear relationship in the range 1.0 to 100 pg mL, and the detection limit is 5 fg mL. When it is applied to the detection of citrinin contents in food sample (i.e., rice and millet) solutions, the RSD is less than 6.1%, and the recoveries for spiked standards range from 95.5 to 102.0%. Hence, this work provides a promising alternative for citrinin detection.
基于分子印迹聚合物和负载 Ru(bpy)和氮掺杂碳量子点 (NCQDs) 的 SiO2 纳米粒子的电致化学发光 (ECL) 传感器被构建用于检测桔青霉素。Ru(bpy) 作为 ECL 发射器，NCQDs 与溶液中的三丙胺 (TPA) 协同作用作为共反应物以促进发光。通过电化学方法将桔青霉素印迹聚（对氨基苯硫酚）膜沉积在发光体表面，除了识别目标外，还可以固定发光体。所获得的 ECL 传感器表现出高灵敏度、稳定性和重现性。ECL 强度的变化与桔青霉素浓度的对数在 1.0 到 100 pg mL 的范围内呈现良好的线性关系，检测限为 5 fg mL。当将其应用于食品样品（即大米和小米）溶液中桔青霉素含量的检测时，RSD 小于 6.1%，加标标准品的回收率在 95.5%至 102.0%之间。因此，这项工作为桔青霉素检测提供了一种有前途的替代方法。
