Runda Michael E, de Kok Niels A W, Schmidt Sandy
Department of Chemical and Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, 9713 AV, Groningen, The Netherlands.
Chembiochem. 2023 Aug 1;24(15):e202300078. doi: 10.1002/cbic.202300078. Epub 2023 Jun 21.
Enzymes that depend on sophisticated electron transfer via ferredoxins (Fds) exhibit outstanding catalytic capabilities, but despite decades of research, many of them are still not well understood or exploited for synthetic applications. This review aims to provide a general overview of the most important Fd-dependent enzymes and the electron transfer processes involved. While several examples are discussed, we focus in particular on the family of Rieske non-heme iron-dependent oxygenases (ROs). In addition to illustrating their electron transfer principles and catalytic potential, the current state of knowledge on structure-function relationships and the mode of interaction between the redox partner proteins is reviewed. Moreover, we highlight several key catalyzed transformations, but also take a deeper dive into their engineerability for biocatalytic applications. The overall findings from these case studies highlight the catalytic capabilities of these biocatalysts and could stimulate future interest in developing additional Fd-dependent enzyme classes for synthetic applications.
依赖铁氧化还原蛋白（Fds）进行复杂电子转移的酶具有出色的催化能力，但尽管经过数十年的研究，其中许多酶仍未得到充分理解，也未被用于合成应用。本综述旨在对最重要的依赖Fd的酶以及所涉及的电子转移过程进行总体概述。虽然讨论了几个例子，但我们特别关注里氏非血红素铁依赖性加氧酶（ROs）家族。除了说明它们的电子转移原理和催化潜力外，还综述了关于结构-功能关系以及氧化还原伴侣蛋白之间相互作用模式的当前知识状态。此外，我们强调了几个关键的催化转化反应，同时也深入探讨了它们在生物催化应用中的可工程化性。这些案例研究的总体结果突出了这些生物催化剂的催化能力，并可能激发未来对开发更多用于合成应用的依赖Fd的酶类的兴趣。
