College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010018, China; Integrative Research Base of Beef and Lamb Processing Technology, Inner Mongolia Agricultural University, Hohhot 010018, China.
Inner Mongolia Vocational College of Chemical Engineering, Hohhot 010010, China.
Ultrason Sonochem. 2023 May;95:106366. doi: 10.1016/j.ultsonch.2023.106366. Epub 2023 Mar 15.
The study evaluated the effect of an ultrasound-assisted treatment on the structural and functional properties of sheep bone collagen (SBC). The type and distribution of SBC were analyzed by proteome (shotgun) technology combined with liquid chromatography-tandem mass spectrometry. Compared with pepsin extraction, the ultrasound-assisted treatment significantly increased the collagen extraction rate by 17.4 pp (P < 0.05). The characteristic functional groups and structural integrity of collagen extracted by both methods were determined via Fourier transform infrared spectroscopy, ultraviolet absorption spectroscopy, and fluorescence spectroscopy. Circular dichroism spectra revealed that the ultrasound-assisted pretreatment reduced α-helix content by 1.6 pp, β-sheet content by 21.9 pp, and random coils content by 28.4 pp, whereas it increased β-turn content by 51.9 pp (P < 0.05), compared with pepsin extraction. Moreover, ultrasound-assisted treatment collagen had superior functional properties (e.g., solubility, water absorption, and oil absorption capacity) and foaming and emulsion properties, compared with pepsin extraction. Furthermore, the relative content of type I collagen in ultrasound-assisted extracted SBC was highest at 79.66%; only small proportions of type II, VI, X, and XI collagen were present. Peptide activity analysis showed that SBC had potential antioxidant activity, dipeptidyl peptidase 4 inhibitory activity, and angiotensin-converting enzyme inhibitory activity; it also had anticancer, antihypertensive, anti-inflammatory, and immunomodulatory effects.
本研究评估了超声辅助处理对绵羊骨胶原（SBC）结构和功能特性的影响。采用蛋白质组学（shotgun）技术结合液相色谱-串联质谱分析 SBC 的类型和分布。与胃蛋白酶提取相比，超声辅助处理使胶原提取率显著提高了 17.4%（P<0.05）。通过傅里叶变换红外光谱、紫外吸收光谱和荧光光谱测定两种方法提取的胶原的特征功能基团和结构完整性。圆二色光谱表明，与胃蛋白酶提取相比，超声辅助预处理使 α-螺旋含量减少了 1.6%，β-折叠含量减少了 21.9%，无规卷曲含量减少了 28.4%，而β-转角含量增加了 51.9%（P<0.05）。此外，与胃蛋白酶提取相比，超声辅助处理胶原具有更优的功能特性（如溶解度、吸水性和吸油性）和泡沫及乳化性能。此外，超声辅助提取 SBC 中 I 型胶原的相对含量最高，为 79.66%；仅存在少量的 II 型、VI 型、X 型和 XI 型胶原。肽活性分析表明，SBC 具有潜在的抗氧化活性、二肽基肽酶 4 抑制活性和血管紧张素转化酶抑制活性；还具有抗癌、降压、抗炎和免疫调节作用。
