Tang Lizong, Zhao Congrui, Liu Yufei, Zhou Jie, Dong Yunsheng, Huang Jiaxing, Yang Tingting, Xiao Hui, Liu Dingbin, Wang Shufang, Cai Hong
Key Laboratory of Bioactive Materials for Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
Key Laboratory of Medicinal Chemical Biology, Research Center for Analytical Sciences, Tianjin Key Laboratory of Molecular Recognition and Biosensing, and Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
ACS Omega. 2023 Mar 8;8(11):10030-10039. doi: 10.1021/acsomega.2c07291. eCollection 2023 Mar 21.
Chronic diabetic wounds have become a significant cause of disability worldwide. It is highly desired to develop effective therapies that can promote the rapid healing of diabetic wounds. Owing to the outstanding hydrophilic and water-retaining properties, hydrogels could accelerate the healing process. Extracellular vesicles (EVs) have shown the ability to promote cell regeneration and angiogenesis. In this study, we chose a gelatin methacryloyl (GelMA) hydrogel, a kind of biomaterial characteristic of good biocompatibility, to load the EVs derived from umbilical cord mesenchymal stem cells (UCMSCs) in order to have a long-lasting effect by consistent release of EVs. Then, the hydrogel with EVs was used to treat diabetic wounds in rat models. Nuclear magnetic resonance spectroscopy and scanning electron microscopy were used to characterize the synthesis of the hydrogel; cell experiments, animal experiments, and histological staining were used to evaluate the function of the hydrogel with EVs. The results show that the GelMA hydrogel incorporated with the UCMSC-derived EVs exhibits unique physicochemical properties, excellent biocompatibility, and much enhanced therapeutic effects for diabetic wounds.
慢性糖尿病伤口已成为全球致残的一个重要原因。人们迫切希望开发出能够促进糖尿病伤口快速愈合的有效疗法。由于具有出色的亲水性和保水性能，水凝胶可以加速愈合过程。细胞外囊泡（EVs）已显示出促进细胞再生和血管生成的能力。在本研究中，我们选择了一种具有良好生物相容性的生物材料——甲基丙烯酰化明胶（GelMA）水凝胶，来负载源自脐带间充质干细胞（UCMSCs）的细胞外囊泡，以便通过细胞外囊泡的持续释放产生持久效果。然后，将含有细胞外囊泡的水凝胶用于治疗大鼠模型中的糖尿病伤口。利用核磁共振光谱和扫描电子显微镜对水凝胶的合成进行表征；通过细胞实验、动物实验和组织学染色来评估含有细胞外囊泡的水凝胶的功能。结果表明，负载有源自脐带间充质干细胞的细胞外囊泡的GelMA水凝胶具有独特的物理化学性质、出色的生物相容性，并且对糖尿病伤口的治疗效果有显著增强。
