Department of Epidemiology and Disease Control, National Veterinary Institute (SVA), Uppsala, Sweden.
Department of Microbiology, Public Health Agency of Sweden, Solna, Sweden.
Front Public Health. 2023 Mar 8;11:1129083. doi: 10.3389/fpubh.2023.1129083. eCollection 2023.
Several Proficiency Test (PT) or External Quality Assessment (EQA) schemes are currently available for assessing the ability of laboratories to detect and characterize enteropathogenic bacteria, but they are usually targeting one sector, covering either public health, food safety or animal health. In addition to sector-specific PTs/EQAs for detection, cross-sectoral panels would be useful for assessment of the capacity to detect and characterize foodborne pathogens in a One Health (OH) perspective and further improving food safety and interpretation of cross-sectoral surveillance data. The aims of the study were to assess the cross-sectoral capability of European public health, animal health and food safety laboratories to detect, characterize and notify findings of the foodborne pathogens spp., spp. and , and to develop recommendations for future cross-sectoral PTs and EQAs within OH. The PT/EQA scheme developed within this study consisted of a test panel of five samples, designed to represent a theoretical outbreak scenario.
A total of 15 laboratories from animal health, public health and food safety sectors were enrolled in eight countries: Denmark, France, Italy, the Netherlands, Poland, Spain, Sweden, and the United Kingdom. The laboratories analyzed the samples according to the methods used in the laboratory and reported the target organisms at species level, and if applicable, serovar for and bioserotype for .
All 15 laboratories analyzed the samples for , 13 for and 11 for . Analytical errors were predominately false negative results. One sample (. Stockholm and O:3/BT4) with lower concentrations of target organisms was especially challenging, resulting in six out of seven false negative results. These findings were associated with laboratories using smaller sample sizes and not using enrichment methods. Detection of was most commonly mandatory to notify within the three sectors in the eight countries participating in the pilot whereas findings of Campylobacter and were notifiable from human samples, but less commonly from animal and food samples.
The results of the pilot PT/EQA conducted in this study confirmed the possibility to apply a cross-sectoral approach for assessment of the joint OH capacity to detect and characterize foodborne pathogens.
目前有几种能力验证（PT）或外部质量评估（EQA）方案可用于评估实验室检测和鉴定肠道致病菌的能力，但这些方案通常针对一个领域，涵盖公共卫生、食品安全或动物健康。除了针对检测的特定领域的 PT/EQA 外，跨领域小组对于从一个健康（OH）的角度评估检测和鉴定食源性病原体的能力以及进一步提高食品安全和解释跨领域监测数据也很有用。本研究的目的是评估欧洲公共卫生、动物健康和食品安全实验室在检测、鉴定和报告食源性病原体 spp.、 spp. 和 spp. 的发现方面的跨领域能力，并为 OH 内未来的跨领域 PT 和 EQA 制定建议。本研究中开发的 PT/EQA 方案包括五个样本的测试小组，旨在代表一个理论爆发场景。
来自动物健康、公共卫生和食品安全领域的 15 个实验室在八个国家（丹麦、法国、意大利、荷兰、波兰、西班牙、瑞典和英国）参加了该方案。实验室根据实验室使用的方法对样本进行分析，并报告目标生物体的种水平，如果适用，还报告 和 的血清型。
所有 15 个实验室都对 进行了分析，13 个实验室对 进行了分析，11 个实验室对 进行了分析。分析错误主要是假阴性结果。一个样本（. Stockholm 和 O:3/BT4）中目标生物体的浓度较低，特别具有挑战性，导致七个结果中有六个是假阴性。这些发现与使用较小样本量且不使用富集方法的实验室有关。在参加试点的八个国家中，三个领域中最常要求通知 的发现，而 Campylobacter 和 的发现仅可从人类样本中报告，但从动物和食品样本中报告的频率较低。
本研究中进行的试点 PT/EQA 的结果证实了应用跨领域方法评估 OH 联合检测和鉴定食源性病原体的能力的可能性。
