Yong Wang, Chunbo Xu, Laga Tong, Xiaoming Zhang, Mingjiu Wang
College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Inner Mongolia Autonomous Region, Hohhot, 010011, China.
Research Center of Grass Germplasm and Genetic Breeding, Institute of Grassland Research of CAAS, Inner Mongolia Autonomous Region, Hohhot, 010019, China.
Open Life Sci. 2023 Mar 21;18(1):20220582. doi: 10.1515/biol-2022-0582. eCollection 2023.
In this study, genetic diversity and germplasm identification of 28 alfalfa germplasm cultivars materials were evaluated by analyzing their internal transcribed spacer 2 (ITS2), L-F, and A-H sequences to provide the innovative reference of alfalfa varieties genetic diversity and identify research. The results showed that the fragment average length of ITS2, L-F, and A-H sorting sequences were 455.7 bp, 230.3 bp, and 345.6 bp, respectively. The ITS2 sequence was too conservative to reflect the individual differences between intercultivars and intracultivars in the preliminary experiment. Furthermore, L-F and A-H sequence differences were relatively small between intercultivars but significant between intracultivars. Alfalfa cultivars were divided into four groups by sequence similarity clustering. Alfalfa cultivars L-F and A-H sequences have apparent differences, showing that chloroplast conservative sequences were independent evolution. Compared with L-F and A-H sequences of alfalfa cultivars, A-H sequence has abundant variation sites and can better reflect the differences between cultivars than the L-F sequence. Therefore, the A-H sequence can identify different alfalfa cultivars and establish the DNA sequence fingerprint.
本研究通过分析28份苜蓿种质品种材料的内转录间隔区2（ITS2）、L-F和A-H序列，对其遗传多样性和种质鉴定进行评估，为苜蓿品种遗传多样性及鉴定研究提供创新性参考。结果表明，ITS2、L-F和A-H排序序列的片段平均长度分别为455.7bp、230.3bp和345.6bp。在初步实验中，ITS2序列过于保守，无法反映品种间和品种内的个体差异。此外，品种间L-F和A-H序列差异相对较小，但品种内差异显著。通过序列相似性聚类将苜蓿品种分为四组。苜蓿品种的L-F和A-H序列存在明显差异，表明叶绿体保守序列是独立进化的。与苜蓿品种的L-F和A-H序列相比，A-H序列具有丰富的变异位点，比L-F序列能更好地反映品种间的差异。因此，A-H序列可用于鉴定不同苜蓿品种并建立DNA序列指纹图谱。
