Makkai Geza, Abraham Istvan M, Barabas Klaudia, Godo Soma, Ernszt David, Kovacs Tamas, Kovacs Gergely, Szocs Szilard, Janosi Tibor Z
Institute of Physiology, Medical School, University of Pécs, Pécs, Hungary.
Nano-Bio-Imaging Core Facility at the Szentágothai Research Centre of the University of Pécs, Pécs, Hungary.
Front Neuroinform. 2023 Mar 8;17:1005936. doi: 10.3389/fninf.2023.1005936. eCollection 2023.
The rapid effects of estradiol on membrane receptors are in the focus of the estradiol research field, however, the molecular mechanisms of these non-classical estradiol actions are poorly understood. Since the lateral diffusion of membrane receptors is an important indicator of their function, a deeper understanding of the underlying mechanisms of non-classical estradiol actions can be achieved by investigating receptor dynamics. Diffusion coefficient is a crucial and widely used parameter to characterize the movement of receptors in the cell membrane. The aim of this study was to investigate the differences between maximum likelihood-based estimation (MLE) and mean square displacement (MSD) based calculation of diffusion coefficients. In this work we applied both MSD and MLE to calculate diffusion coefficients. Single particle trajectories were extracted from simulation as well as from α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor tracking in live estradiol-treated differentiated PC12 (dPC12) cells. The comparison of the obtained diffusion coefficients revealed the superiority of MLE over the generally used MSD analysis. Our results suggest the use of the MLE of diffusion coefficients because as it has a better performance, especially for large localization errors or slow receptor movements.
雌二醇对膜受体的快速作用是雌二醇研究领域的重点，然而，这些非经典雌二醇作用的分子机制尚不清楚。由于膜受体的横向扩散是其功能的重要指标，通过研究受体动力学可以更深入地了解非经典雌二醇作用的潜在机制。扩散系数是表征受体在细胞膜中运动的关键且广泛使用的参数。本研究的目的是研究基于最大似然估计（MLE）和基于均方位移（MSD）计算扩散系数之间的差异。在这项工作中，我们应用MSD和MLE来计算扩散系数。从模拟以及在经雌二醇处理的分化PC12（dPC12）活细胞中对α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）受体的追踪中提取单粒子轨迹。对所得扩散系数的比较揭示了MLE相对于普遍使用的MSD分析的优越性。我们的结果表明应使用扩散系数的MLE，因为它具有更好的性能，特别是对于较大的定位误差或较慢的受体运动。
