用于重组质粒定点诱变的合成寡核苷酸双链体的设计
Design of synthetic oligonucleotide duplexes for site-directed mutagenesis with recombinant plasmids.
作者信息
Sedlácek J, Fábry M, Smrt J
机构信息
Czechoslovak Academy of Sciences, Institute of Molecular Genetics, Prague.
出版信息
Nucleic Acids Symp Ser. 1987(18):245-8.
PMID:3697136
Abstract
Screening for site-mutated plasmids may be greatly facilitated by the different occurrence of the restriction sites distinguishing the altered sequence from the original one. Synthetic oligonucleotides can be so designed that they, apart from defining the mutation, also create a new restriction site recognizable in the restriction pattern of the mutant plasmid.
摘要
通过区分改变序列与原始序列的限制性酶切位点的不同出现情况，可极大地促进对位点突变质粒的筛选。合成寡核苷酸可以这样设计，即它们除了定义突变外，还能在突变体质粒的限制性酶切图谱中产生一个可识别的新限制性酶切位点。
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