Kang Min, Wang Hongxia, Chen Chuxin, Suo Ran, Sun Jianfeng, Yue Quanhong, Liu Yaqiong
College of Food Science and Technology, Hebei Agricultural University, 289th Lingyusi Street, Lianchi District, Baoding 071000, China.
Food Chem X. 2023 Jul 8;19:100786. doi: 10.1016/j.fochx.2023.100786. eCollection 2023 Oct 30.
Organic milk has a high risk of food fraud as it can easily be adulterated with non-organic milk. This study aimed to identify metabolite markers for assessing the authenticity of organic milk from Jersey and Yak. In the untargeted strategy, ultra-high performance liquid chromatography-Q Exactive HF-X mass spectrometer coupled with chemometrics analysis was used to screen and identify tentative markers of organic milk from Jersey and Yak. In the targeted strategy, a quick and easy method of ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was developed to quantify three markers. The peptide of Thr-Ala-Val and D-biotin were determined to be metabolite markers for distinguishing organic and non-organic Jersey milk, whereas trimethylamine -oxide was determined to be a metabolite marker for distinguishing organic and non-organic Yak milk. These findings provide critical information to facilitate assessments of organic milk authenticity.
有机牛奶存在较高的食品欺诈风险，因为它很容易被非有机牛奶掺假。本研究旨在识别代谢物标记物，以评估泽西牛和牦牛有机牛奶的真实性。在非靶向策略中，采用超高效液相色谱-Q Exactive HF-X质谱仪结合化学计量学分析，对泽西牛和牦牛有机牛奶的潜在标记物进行筛选和鉴定。在靶向策略中，开发了一种快速简便的超高效液相色谱-串联质谱法（UPLC-MS/MS）来定量三种标记物。已确定苏氨酸-丙氨酸-缬氨酸肽和D-生物素为区分有机和非有机泽西牛奶的代谢物标记物，而氧化三甲胺则被确定为区分有机和非有机牦牛牛奶的代谢物标记物。这些发现为促进有机牛奶真实性评估提供了关键信息。
