Department of Analytical Chemistry, Faculty of Pharmacy, Erzincan Binali Yildirim University, 25240, Erzincan, Turkey.
Department of Medical Services and Techniques, Çayırlı Vocational School, Erzincan Binali Yildirim University, 24500, Erzincan, Turkey.
Chem Biodivers. 2023 May;20(5):e202201189. doi: 10.1002/cbdv.202201189. Epub 2023 Apr 7.
Geographic conditions (altitude, climate, and local flora) lead to significant differences in the chemical composition of propolis. Therefore, more research is needed for propolis in different geographical regions. So, the aim of this study was to evaluate the phenolic profile, total phenolic content, antioxidant, and antidiabetic properties of Pülümür propolis from Turkey. Methanol (MeOH), chloroform (CHCl ), and hexane extracts of propolis were analyzed. LC-ESI-MS/MS analysis of the extracts showed that the most abundant phenolic compound is caffeic acid in the MeOH extract (2943.12±11.12 μg phenolics/g extract), while on the other hand, CHCl extract had the highest total phenolic content (125.75±1.02 mg GAE/g extract). Antioxidant activity was measured using ABTS and DPPH assays, whereas CHCl extract (IC =6.35±0.11 and 28.84±0.10 μg/mL, respectively) and MeOH extracts (IC =5.04±0.07 and 28.80±0.09 μg/mL, respectively) showed relatively high antioxidant activity. The MeOH extract showed better antidiabetic activity than the standard compound, acarbose (IC =0.544 and 0.805 mg/mL, respectively).
地理条件（海拔、气候和当地植物群）导致蜂胶的化学成分存在显著差异。因此，需要对不同地理区域的蜂胶进行更多的研究。因此，本研究旨在评估来自土耳其的普吕穆尔蜂胶的酚类物质组成、总酚含量、抗氧化和抗糖尿病特性。对蜂胶的甲醇（MeOH）、氯仿（CHCl ）和正己烷提取物进行了分析。提取物的 LC-ESI-MS/MS 分析表明，MeOH 提取物中最丰富的酚类化合物是咖啡酸（2943.12±11.12μg 类黄酮/g 提取物），而另一方面，CHCl 提取物的总酚含量最高（125.75±1.02mg GAE/g 提取物）。使用 ABTS 和 DPPH 测定法测定了抗氧化活性，而 CHCl 提取物（IC =6.35±0.11 和 28.84±0.10μg/mL，分别）和 MeOH 提取物（IC =5.04±0.07 和 28.80±0.09μg/mL，分别）表现出相对较高的抗氧化活性。MeOH 提取物的抗糖尿病活性优于标准化合物阿卡波糖（IC =0.544 和 0.805mg/mL，分别）。
