Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institute, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Mar Drugs. 2023 Feb 25;21(3):155. doi: 10.3390/md21030155.
Marine bivalves are rich in docosahexaenoic acid (DHA), a polyunsaturated fatty acid known to be beneficial for human health; however, the potential role of DHA in protecting shellfish from the toxicity of diarrhetic shellfish toxins (DSTs) remains poorly understood. Here, we aimed to study the effect of DHA on the response of the bivalve, , to DSTs by using LC-MS/MS, RT-qPCR, and histological examination. In this study, we observed that the DHA content decreased significantly with esterification of DSTs in the digestive gland of the mussel after 96 h of exposure to , a DST-producing dinoflagellate. The addition of DHA significantly increased the esterification level of DSTs and increased the expression of Nrf2 signaling pathway-related genes and enzyme activities, alleviating the damage of DSTs to digestive glands. These results suggested that DHA may mediate the esterification of DSTs and activation of the Nrf2 signaling pathway in to protect mussels from the toxic effects of DSTs. This study may provide new insights regarding the response of bivalves to DSTs and lay the foundation for uncovering the role of DHA in environmental adaptation of bivalves.
海洋双壳贝类富含二十二碳六烯酸（DHA），一种多不饱和脂肪酸，已知对人类健康有益；然而，DHA 保护贝类免受腹泻性贝类毒素（DSTs）毒性影响的潜在作用仍知之甚少。在这里，我们旨在通过 LC-MS/MS、RT-qPCR 和组织学检查研究 DHA 对贝类，贻贝 ，对 DSTs 反应的影响。在这项研究中，我们观察到，在暴露于产生 DST 的甲藻 96 小时后，贻贝的消化腺中 DHA 的含量随着 DSTs 的酯化而显著下降。DHA 的添加显著增加了 DSTs 的酯化水平，并增加了 Nrf2 信号通路相关基因和酶活性的表达，减轻了 DSTs 对消化腺的损伤。这些结果表明，DHA 可能介导 DSTs 的酯化和 Nrf2 信号通路在贻贝中的激活，以保护贻贝免受 DSTs 的毒性影响。本研究可能为贝类对 DSTs 的反应提供新的见解，并为揭示 DHA 在贝类环境适应中的作用奠定基础。
