Kajiwara-Kubota Mariko, Uchiyama Kazuhiko, Asaeda Kohei, Kobayashi Reo, Hashimoto Hikaru, Yasuda Takeshi, Sugino Satoshi, Sugaya Takeshi, Hirai Yasuko, Mizushima Katsura, Doi Toshifumi, Inoue Ken, Dohi Osamu, Yoshida Naohisa, Ishikawa Takeshi, Takagi Tomohisa, Konishi Hideyuki, Inoue Ryo, Itoh Yoshito, Naito Yuji
Department of Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
Department of Medical Regulatory Science, Kyoto Prefectural University of Medicine, Kyoto, 602-8566, Japan.
NPJ Sci Food. 2023 Mar 28;7(1):10. doi: 10.1038/s41538-023-00184-4.
Colonic mucus layers protect intestinal tissues against intestinal bacteria. We investigated the effects of dietary fiber and its metabolites on mucus production in the colonic mucosa. Mice were fed a partially hydrolyzed guar gum (PHGG)-containing diet and a fiber-free diet (FFD). The colon mucus layer, fecal short-chain fatty acid (SCFA) levels, and gut microbiota were evaluated. Mucin 2 (MUC2) expression was assessed in SCFA-treated LS174T cells. The role of AKT in MUC2 production was investigated. The mucus layer in the colonic epithelium was significantly increased in the PHGG group compared with that in the FFD group. In the PHGG group, an increase in Bacteroidetes in the stool was observed, and fecal acetate, butyrate, propionate, and succinate levels were significantly increased. However, MUC2 production was significantly increased only in succinate-stimulated LS174T cells. The succinate-induced MUC2 production was associated with AKT phosphorylation. Succinate mediated the PHGG-induced increase in the colon mucus layer.
结肠黏液层可保护肠道组织免受肠道细菌侵害。我们研究了膳食纤维及其代谢产物对结肠黏膜黏液分泌的影响。给小鼠喂食含部分水解瓜尔胶（PHGG）的饮食和无纤维饮食（FFD）。评估结肠黏液层、粪便短链脂肪酸（SCFA）水平和肠道微生物群。在经SCFA处理的LS174T细胞中评估黏蛋白2（MUC2）的表达。研究了AKT在MUC2产生中的作用。与FFD组相比，PHGG组结肠上皮中的黏液层显著增加。在PHGG组中，观察到粪便中拟杆菌增加，粪便中乙酸盐、丁酸盐、丙酸盐和琥珀酸盐水平显著升高。然而，仅在琥珀酸盐刺激的LS174T细胞中MUC2产生显著增加。琥珀酸盐诱导的MUC2产生与AKT磷酸化有关。琥珀酸盐介导了PHGG诱导的结肠黏液层增加。
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