Matos Alice, Cunha Eva, Baptista Lara, Tavares Luís, Oliveira Manuela
Veterinários Sem Fronteiras, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal.
CIISA-Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal.
Antibiotics (Basel). 2023 Feb 23;12(3):447. doi: 10.3390/antibiotics12030447.
Antimicrobial resistance is a public health threat with an increasing expression in low- and middle-income countries such as Cape Verde. In this country, there is an overpopulation of dogs, which may facilitate the spread of resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae. To clarify the role of dogs as reservoirs for the dissemination of this bacterial group, 100 rectal swab samples were collected from confined (n = 50) and non-confined (n = 50) dogs in Santiago and Boa Vista Islands, Cape Verde. These were analyzed using conventional bacteriological techniques for the detection of ESBL-producing Enterobacteriaceae and characterization of their pathogenic and resistance profiles. Twenty-nine samples displayed ESBL-positive bacteria, from which 48 ESBL-producing isolates were obtained and mostly identified as . Multiple antimicrobial resistance indexes ranged from 0.18 to 0.70 and half of the isolates were classified as multidrug-resistant. Isolates were capable of producing relevant virulence factors, including biofilm, showing virulence indexes between 0.29 and 0.71. As such, dogs in Cape Verde may act as reservoirs of resistant bacteria, including pathogenic and zoonotic species, representing a public health concern. Although further investigation is needed, this study proposes the periodical analysis of dogs' fecal samples to monitor resistance dissemination in the country, in a One-Health perspective.
抗菌药物耐药性是一种公共卫生威胁，在佛得角等低收入和中等收入国家的表现日益突出。在该国，狗的数量过多，这可能会促进耐药菌的传播，包括产超广谱β-内酰胺酶（ESBL）的肠杆菌科细菌。为了阐明狗作为该菌群传播宿主的作用，从佛得角圣地亚哥岛和博阿维斯塔岛的圈养犬（n = 50）和非圈养犬（n = 50）中采集了100份直肠拭子样本。使用传统细菌学技术对这些样本进行分析，以检测产ESBL的肠杆菌科细菌，并对其致病性和耐药性特征进行表征。29份样本显示出ESBL阳性细菌，从中获得了48株产ESBL的分离株，大多被鉴定为 。多重抗菌药物耐药指数在0.18至0.70之间，一半的分离株被归类为多重耐药。分离株能够产生相关毒力因子，包括生物膜，毒力指数在0.29至0.71之间。因此，佛得角的狗可能充当耐药菌的宿主，包括致病性和人畜共患病菌，这是一个公共卫生问题。尽管需要进一步调查，但本研究从“同一健康”的角度建议定期分析狗的粪便样本，以监测该国的耐药性传播情况。
