Ferrer-Luque Carmen Maria, Solana Carmen, Aguado Beatriz, Baca Pilar, Arias-Moliz María Teresa, Ruiz-Linares Matilde
Department of Stomatology, School of Dentistry, University of Granada, Granada, Spain.
Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.
Aust Endod J. 2023 Dec;49(3):530-536. doi: 10.1111/aej.12776. Epub 2023 Jul 18.
The objective of this research was to evaluate the efficacy of diclofenac sodium solutions, with or without cetrimide (CTR) added, against polymicrobial root canal biofilms grown in dentin specimens. The study groups were: (1) 5% diclofenac sodium (DCS); (2) 2.5% DCS; (3) 2.5% DCS + 0. 2% CTR; (4) 2.5% DCS + 0.4% CTR and (5) 0.9% saline solution (SS) as the control. After 5 min of solution contact with the biofilms, the antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay as well as confocal laser scanning microscopy (CLSM). Microbial quantification was indicated as the percentage reduction of relative light units (RLUs) for the ATP assay, the Log total biovolume and the viability percentage (green cells) for CLSM. Solutions of 2.5% DCS + 0.4% CTR and 5% DCS showed the highest antimicrobial efficacy. Cetrimide increased the antibiofilm activity of diclofenac sodium against endodontic biofilms.
本研究的目的是评估添加或不添加西曲溴铵（CTR）的双氯芬酸钠溶液对牙本质标本中生长的多微生物根管生物膜的疗效。研究组包括：（1）5%双氯芬酸钠（DCS）；（2）2.5% DCS；（3）2.5% DCS + 0.2% CTR；（4）2.5% DCS + 0.4% CTR 和（5）0.9%盐溶液（SS）作为对照。溶液与生物膜接触5分钟后，通过三磷酸腺苷（ATP）检测以及共聚焦激光扫描显微镜（CLSM）评估抗菌活性。微生物定量在ATP检测中以相对光单位（RLU）的减少百分比表示，在CLSM中以总生物体积对数和活力百分比（绿色细胞）表示。2.5% DCS + 0.4% CTR溶液和5% DCS溶液显示出最高的抗菌效果。西曲溴铵增强了双氯芬酸钠对牙髓生物膜的抗生物膜活性。
