Kuppusamy Palaniselvam, Kim Sujung, Kim Sung-Jo, Park Myunghum, Song Ki-Duk
Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea.
Division of Cosmetics and Biotechnology, College of Life and Health Sciences, Hoseo University, Asan 31499, Republic of Korea.
Antibiotics (Basel). 2023 Mar 3;12(3):507. doi: 10.3390/antibiotics12030507.
Biological synthesis of metal nanoparticles has a significant impact in developing sustainable technologies for human, animal, and environmental safety. In this study, we synthesized gold and silver nanoparticles (NPs) using (SP) extract and characterized them using UV-visible spectrophotometry, FESEM-EDX, HR-TEM, XRD, and FT-IR spectroscopy. Furthermore, antimicrobial and antioxidant activities and cytotoxicity of the synthesized NPs were evaluated. UV-visible absorption spectra showed λ at 531 and 410 nm, corresponding to the presence of SP gold NPs (SP-AuNPs) and SP silver NPs (SP-AgNPs). Most NPs were spherical and a few were triangular rods, measuring 5-30 and 10-40 nm, respectively. EDX elemental composition analysis revealed that SP-AuNPs and SP-AgNPs accounted for >60% and 30% of NPs, respectively. Additionally, some organic moieties were present, likely derived from various metabolites in the natural plant extract, which acted as stabilizing and reducing agents. Next, the antimicrobial activity of the NPs against pathogenic microbes was tested. SP-AgNPs showed potent antibacterial activity against and . Moreover, at moderate and low concentrations, both NPs exhibited weak cytotoxicity in chicken fibroblasts (DF-1) and macrophages (HD11) as well as human intestinal cancer cells (HT-29). Meanwhile, at high concentrations, the NPs exhibited strong cytotoxicity in both chicken and human cell lines. Therefore, the synthesized SP-AuNPs and SP-AgNPs may act as promising materials to treat poultry diseases.
金属纳米粒子的生物合成对开发保障人类、动物和环境安全的可持续技术具有重大影响。在本研究中，我们使用（某种植物）提取物合成了金纳米粒子和银纳米粒子（NPs），并通过紫外可见分光光度法、场发射扫描电子显微镜-能谱仪（FESEM-EDX）、高分辨率透射电子显微镜（HR-TEM）、X射线衍射仪（XRD）和傅里叶变换红外光谱仪（FT-IR）对其进行了表征。此外，还评估了合成纳米粒子的抗菌、抗氧化活性及细胞毒性。紫外可见吸收光谱显示，在531和410 nm处有吸收峰，分别对应（某种植物）金纳米粒子（SP-AuNPs）和（某种植物）银纳米粒子（SP-AgNPs）的存在。大多数纳米粒子呈球形，少数为三角形棒状，尺寸分别为5-30 nm和10-40 nm。能谱仪元素组成分析表明，SP-AuNPs和SP-AgNPs分别占纳米粒子的60%以上和30%。此外，还存在一些有机部分，可能源自天然植物提取物中的各种代谢产物，它们起到了稳定和还原剂的作用。接下来，测试了纳米粒子对致病微生物的抗菌活性。SP-AgNPs对（某些菌株）显示出强大的抗菌活性。此外，在中低浓度下，两种纳米粒子在鸡成纤维细胞（DF-1）、巨噬细胞（HD11）以及人肠道癌细胞（HT-29）中均表现出较弱的细胞毒性。同时，在高浓度下，纳米粒子在鸡和人类细胞系中均表现出较强的细胞毒性。因此，合成的SP-AuNPs和SP-AgNPs可能是治疗家禽疾病的有前景的材料。
