介质阻挡放电等离子体对食品接触表面混合培养生物膜的影响。

Effect of Dielectric Barrier Discharge Plasma against Mixed-Culture Biofilms on Food-Contact Surfaces.

作者信息

Song Min Gyu, Roy Pantu Kumar, Jeon Eun Bi, Kim So Hee, Heu Min Soo, Lee Jung-Suck, Choi Jae-Suk, Kim Jin-Soo, Park Shin Young

机构信息

Institute of Marine Industry, Department of Seafood Science and Technology, Gyeongsang National University, Tongyeong 53064, Republic of Korea.

Research Center for Industrial Development of Seafood, Gyeongsang National University, Tongyeong 53064, Republic of Korea.

出版信息

Antibiotics (Basel). 2023 Mar 19;12(3):609. doi: 10.3390/antibiotics12030609.

is a major foodborne pathogen. Various methods can be used to control biofilms formed by foodborne pathogens. Recently, the food industry has become interested in plasma, which can be used as a non-thermal technology with minimum changes to product quality. In this study, the effects of dielectric barrier discharge (DBD) plasma on mixed-culture biofilms formed on stainless steel (SS), latex hand glove (HG), and silicone rubber (SR) were investigated. DBD plasma effectuated reductions of 0.11-1.14, 0.28-1.27 and 0.37-1.55 log CFU/cm, respectively. Field emission scanning electron microscopy (FE-SEM) demonstrated that DBD plasma cuts off intercellular contact and induces cell decomposition to prevent the development of biological membranes. It was confirmed that the formed biofilms collapsed and separated into individual bacteria. Our findings suggest that DBD plasma can be used as an alternative non-heating sterilization technology in the food industry to reduce biofilm formation on bacterial targets.

是一种主要的食源性病原体。可以使用各种方法来控制食源性病原体形成的生物膜。最近，食品工业对等离子体产生了兴趣，它可以用作对产品质量影响最小的非热技术。在本研究中，研究了介质阻挡放电（DBD）等离子体对在不锈钢（SS）、乳胶手套（HG）和硅橡胶（SR）上形成的混合培养生物膜的影响。DBD等离子体分别实现了0.11 - 1.14、0.28 - 1.27和0.37 - 1.55 log CFU/cm的减少。场发射扫描电子显微镜（FE-SEM）表明，DBD等离子体切断细胞间接触并诱导细胞分解，以防止生物膜的形成。证实形成的生物膜坍塌并分离成单个细菌。我们的研究结果表明，DBD等离子体可以用作食品工业中的替代非加热杀菌技术，以减少细菌靶标上的生物膜形成。