Roy Pantu Kumar, Song Min Gyu, Park Shin Young
Department of Seafood Science and Technology, Institute of Marine Industry, Gyeongsang National University, Tongyeong 53064, Korea.
Foods. 2022 Mar 28;11(7):977. doi: 10.3390/foods11070977.
Quercetin is an active nutraceutical element that is found in a variety of foods, vegetables, fruits, and other products. Due to its antioxidant properties, quercetin is a flexible functional food that has broad protective effects against a wide range of infectious and degenerative disorders. As a result, research is required on food-contact surfaces (rubber (R) and hand gloves (HG)) that can lead to cross-contamination. In this investigation, the inhibitory effects of quercetin, an antioxidant and antibacterial molecule, were investigated at sub-MIC (125; 1/2, 62.5; 1/4, and 31.25; 1/8 MIC, μg/mL) against Salmonella Typhimurium on surfaces. When quercetin (0−125 μg/mL) was observed on R and HG surfaces, the inhibitory effects were 0.09−2.49 and 0.20−2.43 log CFU/cm2, respectively (p < 0.05). The results were confirmed by field emission scanning electron microscopy (FE-SEM), because quercetin inhibited the biofilms by disturbing cell-to-cell connections and inducing cell lysis, resulting in the loss of normal cell morphology, and the motility (swimming and swarming) was significantly different at 1/4 and 1/2 MIC compared to the control. Quercetin significantly (p < 0.05) suppressed the expression levels of virulence and stress response (rpoS, avrA, and hilA) and quorum-sensing (luxS) genes. Our findings imply that plant-derived quercetin could be used as an antibiofilm agent in the food industry to prevent S. Typhimurium biofilm formation.
槲皮素是一种活性营养成分，存在于多种食物、蔬菜、水果及其他产品中。由于其抗氧化特性，槲皮素是一种具有多种功能的食品，对多种传染性和退行性疾病具有广泛的保护作用。因此，需要对可能导致交叉污染的食品接触表面（橡胶（R）和手套（HG））进行研究。在本研究中，研究了抗氧化和抗菌分子槲皮素在亚最低抑菌浓度（125；1/2，62.5；1/4，31.25；1/8最低抑菌浓度，μg/mL）下对表面鼠伤寒沙门氏菌的抑制作用。当在R和HG表面观察到槲皮素（0−125μg/mL）时，抑制效果分别为0.09−2.49和0.20−2.43 log CFU/cm2（p<0.05）。场发射扫描电子显微镜（FE-SEM）证实了该结果，因为槲皮素通过干扰细胞间连接并诱导细胞裂解来抑制生物膜，导致正常细胞形态丧失，并且在1/4和1/2最低抑菌浓度下，与对照相比，运动性（游动和群集）有显著差异。槲皮素显著（p<0.05）抑制了毒力和应激反应（rpoS、avrA和hilA）以及群体感应（luxS）基因的表达水平。我们的研究结果表明，植物来源的槲皮素可作为食品工业中的一种抗生物膜剂，以防止鼠伤寒沙门氏菌生物膜的形成。
