Thomas Som V, Dienger-Stambaugh Krista, Jordan Michael, Wang Yuxin, Hammonds Jason, Spearman Paul, Shi Donglu
Department of Biomedical Engineering, College of Engineering and Applied Science, University of Cincinnati, Cincinnati, OH 45221, USA.
Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center and University of Cincinnati, Cincinnati, OH 45229, USA.
Bioengineering (Basel). 2023 Feb 21;10(3):280. doi: 10.3390/bioengineering10030280.
A Cold Atmospheric Plasma (CAP) apparatus was designed and developed for SARS-CoV-2 killing as evaluated by pseudotyped viral infectivity assays. The reactive species generated by the plasma system was fully characterized by using Optical Emission Spectroscopy (OES) measurement under given conditions such as plasma power, flow rate, and treatment time. A variety of reactive oxygen species (ROS) and reactive nitrogen species (RNS) were identified from plasma plume with energies of 15-72 eV in the frequency range between 500-1000 nm. Systematic virus killing experiments were carried out, and the efficacy of CAP treatment in reducing SARS-CoV-2 viral infectivity was significant following treatment for 8 s, with further enhancement of killing upon longer exposures of 15-120 s. We correlated killing efficacy with the reactive species in terms of type, intensity, energy, and frequency. These experimental results demonstrate effective cold plasma virus killing via ROS and RNS under ambient conditions.
设计并开发了一种用于杀灭新型冠状病毒（SARS-CoV-2）的冷大气等离子体（CAP）装置，通过假型病毒感染性试验进行评估。在给定条件下，如等离子体功率、流速和处理时间，利用发射光谱（OES）测量对等离子体系统产生的活性物质进行了全面表征。在500-1000nm频率范围内，从能量为15-72eV的等离子体羽流中识别出多种活性氧（ROS）和活性氮（RNS）。进行了系统的病毒杀灭实验，CAP处理8秒后，在降低SARS-CoV-2病毒感染性方面效果显著，在15-120秒的更长暴露时间后杀灭效果进一步增强。我们从类型、强度、能量和频率方面将杀灭效果与活性物质进行了关联。这些实验结果表明，在环境条件下，通过ROS和RNS可有效实现冷等离子体病毒杀灭。
