University of Castilla-La Mancha, Institute of Energy Research (INEI), C/Moledores s/n., 13071, Ciudad Real, Spain.
Mechanical Engineering Department, ICAI, Comillas Pontifical University, Alberto Aguilera 25, 28015, Madrid, Spain.
Sci Rep. 2022 Jan 7;12(1):263. doi: 10.1038/s41598-021-04360-y.
Cold Atmospheric Plasma (CAP) and Plasma Activated Media (PAM) are effective against bacteria, fungi, cancer cells, and viruses because they can deliver Reactive Oxygen and Nitrogen Species (RONS) on a living tissue with negligible damage on health cells. The antiviral activity of CAP against SARS-CoV-2 is being investigated, however, the same but of PAM has not been explored despite its potential. In the present study, the capability of Plasma Activated Media (PAM) to inactivate SARS-CoV-2 and PR8 H1N1 influenza virus with negligible damage on healthy cells is demonstrated. PAM acted by both virus detaching and diminished replication. Furthermore, the treatment of A549 lung cells at different times with buffered PAM did not induce interleukin 8 expression, showing that PAM did not induce inflammation. These results open a new research field by using PAM to the development novel treatments for COVID-19, influenza, and other respiratory diseases.
冷等离体气体（CAP）和等离子体激活媒质（PAM）对细菌、真菌、癌细胞和病毒有效，因为它们可以在活体组织上输送活性氧和氮物种（RONS），而对健康细胞几乎没有损伤。CAP 对 SARS-CoV-2 的抗病毒活性正在被研究，然而，尽管有潜力，但 PAM 的相同效果尚未被探索。在本研究中，证明了等离子体激活媒质（PAM）可以在不损伤健康细胞的情况下灭活 SARS-CoV-2 和 PR8 H1N1 流感病毒。PAM 通过病毒脱落和复制减少来起作用。此外，用缓冲的 PAM 不同时间处理 A549 肺细胞不会诱导白细胞介素 8 的表达，表明 PAM 不会引起炎症。这些结果通过使用 PAM 为 COVID-19、流感和其他呼吸道疾病开发新的治疗方法开辟了一个新的研究领域。
