Sedeek Abdelrahman M, Salah Israa, Kamel Hasnaa L, Soltan Mohamed A, Nour Eman, Alshammari Abdulrahman, Riaz Rajoka Muhammad Shahid, Elsayed Tarek R
Department of Microbiology and Immunology, Faculty of Pharmacy, Sinai University-Kantara Branch, Ismailia 41522, Egypt.
Faculty of Pharmacy, Modern University for Technology and Information (MTI), Cairo 11585, Egypt.
Biology (Basel). 2023 Feb 21;12(3):345. doi: 10.3390/biology12030345.
are factories of antimicrobial secondary metabolites. We isolated a species associated with the rhizosphere. Its total metabolic extract exhibited potent antibacterial and antifungal properties against all the tested pathogenic microbes. Whole genome sequencing and genome analyses were performed to take a look at its main characteristics and to reconstruct the metabolic pathways that can be associated with biotechnologically useful traits. AntiSMASH was used to identify the secondary metabolite gene clusters. In addition, we searched for known genes associated with plant growth-promoting characteristics. Finally, a comparative and pan-genome analysis with three closely related genomes was conducted. It was identified as strain AC-40. Genome mining indicated the presence of several secondary metabolite gene clusters. Some of them are identical or homologs to gene clusters of known metabolites with antimicrobial, antioxidant, and other bioactivities. It also showed the presence of several genes related to plant growth promotion traits. The comparative genome analysis indicated that at least five of these gene clusters are highly conserved through rochei group genomes. The genotypic and phenotypic characteristics of strain AC-40 indicate that it is a promising source of beneficial secondary metabolites with pharmaceutical and biotechnological applications.
是抗菌次生代谢物的工厂。我们分离出一种与根际相关的物种。其总代谢提取物对所有测试的致病微生物均表现出强大的抗菌和抗真菌特性。进行了全基因组测序和基因组分析，以了解其主要特征，并重建可能与生物技术有用性状相关的代谢途径。使用AntiSMASH来识别次生代谢物基因簇。此外，我们搜索了与促进植物生长特性相关的已知基因。最后，对三个密切相关的基因组进行了比较和泛基因组分析。它被鉴定为AC-40菌株。基因组挖掘表明存在几个次生代谢物基因簇。其中一些与具有抗菌、抗氧化和其他生物活性的已知代谢物的基因簇相同或同源。它还显示出存在几个与促进植物生长性状相关的基因。比较基因组分析表明，这些基因簇中至少有五个在罗氏菌群基因组中高度保守。AC-40菌株的基因型和表型特征表明，它是具有制药和生物技术应用的有益次生代谢物的有前途的来源。
