Department of Microbiology and Immunology, Faculty of Pharmacy, Sinai University, Ismaillia, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
Lett Appl Microbiol. 2022 Sep;75(3):511-525. doi: 10.1111/lam.13728. Epub 2022 May 9.
Marine micro-organisms are a promising source for novel natural compounds with many medical and biotechnological applications. Here, we demonstrate limitations and recent strategies for investigating the marine microbial community for novel bioactive metabolites, specifically those of antimicrobial potential. These strategies include culture-dependent methods such as modifying the standard culture media, including changing the gelling agent, dissolving vehicle, media supplementation and preparation to access a broader range of bacterial diversity from marine samples. Furthermore, we discuss strategies such as in situ cultivation, dilution-to-extinction cultivation and long-term incubation. We are presenting recent applications of culture-independent methods such as genome mining, proteomics profiling and the application of metagenomics as a novel strategy for structure confirmation in the discovery of the marine micro-organism for novel antimicrobial metabolites. We present this review as a simple guide and a helpful resource for those who seek to enter the challenging field of applied marine microbiology.
海洋微生物是具有许多医学和生物技术应用的新型天然化合物的有前途的来源。在这里，我们展示了用于研究海洋微生物群落中新型生物活性代谢物（特别是具有抗菌潜力的代谢物）的方法的局限性和最新策略。这些策略包括依赖培养的方法，例如修改标准培养基，包括改变凝胶剂、溶解载体、培养基补充和制备，以从海洋样本中获得更广泛的细菌多样性。此外，我们还讨论了原位培养、稀释至灭绝培养和长期孵育等策略。我们介绍了依赖培养的方法的最新应用，如基因组挖掘、蛋白质组学分析和宏基因组学的应用，作为一种新的策略，用于在发现具有新型抗菌代谢物的海洋微生物时对结构进行确认。我们提供本综述作为一个简单的指南和有价值的资源，供那些希望进入应用海洋微生物学这一具有挑战性领域的人参考。
