氚水损伤的永生性人角质形成细胞HaCaT细胞系的转录组分析
Transcriptome Analysis of the Immortal Human Keratinocyte HaCaT Cell Line Damaged by Tritiated Water.
作者信息
Zhang Yan, Zhou Yuanyuan, Wu Hui, Yan Zhuna, Chen Jinwu, Song Wencheng
机构信息
Anhui Province Key Laboratory of Medical Physics and Technology, Institute of Health & Medical Technology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.
School of Life Science, Hefei Normal University, Hefei 230061, China.
出版信息
Biology (Basel). 2023 Mar 3;12(3):405. doi: 10.3390/biology12030405.
Radioactive elements, such as tritium, have been released into the ocean in large quantities as a result of the reactor leakage accident. In this study, an MTT assay demonstrated that the viability of HacaT cells decreased after tritiated water treatment. Bioinformatics analysis was used to analyze gene changes in the HacaT cells. The sequencing results showed 267 significantly differentially expressed genes (DEGs), and GO enrichment analysis showed that the DEGs were mainly divided into three parts. The KEGG pathway analysis showed that the up-regulated DEGs were involved in Wnt and other pathways, while the down-regulated DEGs were involved in Jak-STAT and others. A Western blot assay was used to verify the parts of the sequencing results. This study was the first to explore the mechanism of tritiated water on HacaT cells using Transcriptome analysis. The results will provide a theoretical basis for the study of tritiated water hazard mechanisms.
由于反应堆泄漏事故，氚等放射性元素大量释放到海洋中。在本研究中，MTT 试验表明，经氚水处理后，HacaT 细胞的活力下降。采用生物信息学分析方法分析 HacaT 细胞中的基因变化。测序结果显示有 267 个显著差异表达基因（DEG），基因本体（GO）富集分析表明这些差异表达基因主要分为三个部分。京都基因与基因组百科全书（KEGG）通路分析表明，上调的差异表达基因参与 Wnt 等通路，而下调的差异表达基因参与 Jak-STAT 等通路。采用蛋白质免疫印迹法对部分测序结果进行验证。本研究首次利用转录组分析探索氚水对 HacaT 细胞的作用机制。研究结果将为氚水危害机制的研究提供理论依据。
Zotero 插件