基于 AIEgen 纳米球标记的适体与磁性金纳米复合材料夹心结构检测甲胎蛋白。
Detection of Alpha Fetoprotein Based on AIEgen Nanosphere Labeled Aptamer Combined with Sandwich Structure of Magnetic Gold Nanocomposites.
机构信息
Department of Kidney Transplantation, The Second Xiangya Hospital of Central South University, Changsha 410011, China.
School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
出版信息
Biosensors (Basel). 2023 Mar 6;13(3):351. doi: 10.3390/bios13030351.
As a biomarker, alpha-fetoprotein (AFP) is valuable for detecting some tumors in men, non-pregnant women, and children. However, the detection sensitivity in some methods needs to be improved. Therefore, developing a simple, reliable, and sensitive detection method for AFP is important for non-malignant diseases. An aptamer binding was developed based on aggregation-induced emission luminogen (AIEgen) nanosphere labeled with Fe3O4@MPTMS@AuNPs. AFP was detected with a sandwich structure of AuNPs magnetic composite particles. An aggregation-induced emission (AIE) molecule and polystyrene (PS) nanosphere complex were assembled, enhancing the fluorescence and improving the sensitivity of detection. The limit of detection (LOD) was at a given level of 1.429 pg/mL, which can best be achieved in serum samples. Finally, the results obtained showed the complex to be promising in practical applications.
作为一种生物标志物，甲胎蛋白（AFP）对于检测男性、非孕妇和儿童中的某些肿瘤具有重要价值。然而，一些方法的检测灵敏度需要提高。因此，开发一种简单、可靠和敏感的 AFP 检测方法对于非恶性疾病非常重要。本研究基于基于聚集诱导发射发光体（AIEgen）纳米球标记的四氧化三铁@MPTMS@AuNPs 开发了适体结合物。通过 AuNPs 磁性复合粒子的三明治结构检测 AFP。组装了聚集诱导发射（AIE）分子和聚苯乙烯（PS）纳米球复合物，增强了荧光并提高了检测的灵敏度。检测限（LOD）在 1.429 pg/mL 达到给定水平，在血清样本中可以达到最佳效果。最后，结果表明该复合物在实际应用中具有广阔的前景。
Zotero 插件