College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
Shandong Dyne Marine Biopharmaceutical Co., Ltd., Weihai 264300, China.
Biosensors (Basel). 2023 Mar 9;13(3):361. doi: 10.3390/bios13030361.
Hydrogen peroxide (HO) is an important reactive oxygen species that mediates a variety of physiological functions in biological processes, and it is an essential mediator in food, pharmaceutical, and environmental analysis. However, HO can be dangerous and toxic at certain concentrations. It is crucial to detect the concentration of HO in the environment for human health and environmental protection. Herein, we prepared the red-emitting copper nanoclusters (Cu NCs) by a one-step method, with lipoic acid (LA) and sodium borohydride as protective ligands and reducing agents, respectively, moreover, adding chitosan (CS) to wrap LA-Cu NCs. The as-prepared LA-Cu NCs@CS have stronger fluorescence than LA-Cu NCs. We found that the presence of HO causes the fluorescence of LA-Cu NCs@CS to be strongly quenched. Based on this, a fluorescent probe based on LA-Cu NCs@CS was constructed for the detection of HO with a limit of detection of 47 nM. The results from this research not only illustrate that the as--developed fluorescent probe exhibits good selectivity and high sensitivity to HO in environmental water samples but also propose a novel strategy to prepare red-emitting copper nanoclusters (Cu NCs) by a one-step method.
过氧化氢（HO）是一种重要的活性氧物质，介导着生物过程中的各种生理功能，它是食品、制药和环境分析中必不可少的介质。然而，在某些浓度下，HO 可能是危险和有毒的。因此，检测环境中 HO 的浓度对于人类健康和环境保护至关重要。在此，我们通过一步法制备了红色发射的铜纳米团簇（Cu NCs），分别使用硫辛酸（LA）和硼氢化钠作为保护配体和还原剂，并且添加壳聚糖（CS）来包裹 LA-Cu NCs。所制备的 LA-Cu NCs@CS 比 LA-Cu NCs 具有更强的荧光。我们发现 HO 的存在会导致 LA-Cu NCs@CS 的荧光被强烈猝灭。基于这一点，构建了基于 LA-Cu NCs@CS 的荧光探针，用于检测环境水样中的 HO，其检测限为 47 nM。这项研究的结果不仅表明所开发的荧光探针对环境水样中的 HO 具有良好的选择性和高灵敏度，而且还提出了一种通过一步法制备红色发射铜纳米团簇（Cu NCs）的新策略。
