Zhang Ruizhong, He Shuijian, Zhang Chunmei, Chen Wei
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, China.
J Mater Chem B. 2015 May 28;3(20):4146-4154. doi: 10.1039/c5tb00413f. Epub 2015 Apr 24.
In this study, a simple and one-pot pyrolysis strategy is developed for the mass production of Fe, N-incorporated carbon nanotubes in situ grown on 3D porous carbon foam (denoted as Fe-Phen-CFs), which provides highly active Fe-N and doped-N species, and a large surface area with exposed active sites. The obtained composite exhibits intrinsic peroxidase-like catalytic activities. With the Fe-Phen-CFs as the catalyst, the peroxidase substrate of terephthalic acid (TA) can be oxidized to the fluorescent product of hydroxyterephthalate (HTA) by HO, which provides a unique strategy for fluorescence detection of HO. With such a process, as low as 68 nM HO could be detected with a linear range from 0.1 to 100 μM. Meanwhile, by integrating glucose oxidase on the Fe-Phen-CFs composite, sensitive detection of glucose is also achieved with a linear range from 0.5 to 200 μM and a limit of detection of 0.19 μM. Most importantly, such a novel TA/Fe-Phen-CFs system can be successfully applied to glucose determination in real human serum samples. The unique nature and 3D structure of the Fe-Phen-CFs composite makes it promising for the fabrication of low-cost, high-performance biosensors.
在本研究中，开发了一种简单的一锅热解策略，用于大规模生产原位生长在三维多孔碳泡沫上的铁、氮共掺杂碳纳米管（记为Fe-Phen-CFs），其提供了高活性的Fe-N和掺杂N物种以及具有暴露活性位点的大表面积。所获得的复合材料表现出固有的过氧化物酶样催化活性。以Fe-Phen-CFs作为催化剂，对苯二甲酸（TA）的过氧化物酶底物可被HO氧化为羟基对苯二甲酸（HTA）的荧光产物，这为HO的荧光检测提供了一种独特的策略。通过这样的过程，低至68 nM的HO能够被检测到，线性范围为0.1至100 μM。同时，通过将葡萄糖氧化酶整合到Fe-Phen-CFs复合材料上，也实现了对葡萄糖的灵敏检测，线性范围为0.5至200 μM，检测限为0.19 μM。最重要的是，这种新型的TA/Fe-Phen-CFs系统能够成功应用于实际人血清样品中的葡萄糖测定。Fe-Phen-CFs复合材料独特的性质和三维结构使其在制造低成本、高性能生物传感器方面具有前景。
