Chou Yung-Che, Er Tze-Kiong
Division of Laboratory Medicine, Asia University Hospital, Asia University, Taichung 413, Taiwan.
Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung 413, Taiwan.
Biomedicines. 2023 Mar 22;11(3):986. doi: 10.3390/biomedicines11030986.
The adoption of an automated system can decrease the hands-on time requirements in a clinical laboratory setting. For the detection of HLA-B27, implementing a high-throughput and fully automated system has several advantages over using manual methods. Therefore, this study aimed to evaluate automation efficiency for the detection of HLA-B27. Peripheral blood samples were obtained from 50 participants, and DNA was isolated from these samples. A Pharmigene PG27 detection kit was used for the qualitative detection of HLA-B27. The performances of the semi-automated and fully automated LabTurbo AIO systems in the detection of HLA-B27 were compared. The mean absorbance (optical density) values for the Maelstrom 8 and LabTurbo AIO systems were found to be 1.88 and 1.9, respectively. The housekeeping gene was amplified and quantified using a real-time PCR assay across all DNA extracts to check the quality of the extracted human DNA. The results were expressed as the cycle threshold (Ct) values for all DNA extracts from both platforms. The mean Ct values for the Roche Cobas z480 and LabTurbo AIO systems were found to be 22.7 and 20.4, respectively. This study demonstrated that the semi-automated method and the LabTurbo AIO system yield consistent results for the detection of HLA-B*27. However, compared to the semi-automated method, the LabTurbo AIO system provides standardized procedures, avoids manual handling, and improves turnaround time.
采用自动化系统可以减少临床实验室环境中的实际操作时间要求。对于HLA - B27的检测，与使用手工方法相比，实施高通量全自动系统具有多个优势。因此，本研究旨在评估HLA - B27检测的自动化效率。从50名参与者中采集外周血样本，并从这些样本中分离DNA。使用Pharmigene PG27检测试剂盒对HLA - B27进行定性检测。比较了半自动和全自动LabTurbo AIO系统在检测HLA - B27方面的性能。发现Maelstrom 8和LabTurbo AIO系统的平均吸光度（光密度）值分别为1.88和1.9。使用实时PCR测定法对所有DNA提取物中的管家基因进行扩增和定量，以检查提取的人类DNA的质量。结果以两个平台所有DNA提取物的循环阈值（Ct）值表示。发现罗氏Cobas z480和LabTurbo AIO系统的平均Ct值分别为22.7和20.4。本研究表明，半自动方法和LabTurbo AIO系统在检测HLA - B*27方面产生一致的结果。然而，与半自动方法相比，LabTurbo AIO系统提供标准化程序，避免手工操作，并缩短周转时间。
