Lindner Cordula, Looijesteijn Ellen, Dijck Helmie van, Bovee-Oudenhoven Ingeborg, Heerikhuisen Margreet, Broek Tim J van den, Marzorati Massimo, Triantis Vassilis, Nauta Arjen
FrieslandCampina, 3818 LA Amersfoort, The Netherlands.
The Netherlands Organization for Applied Scientific Research (TNO), Microbiology and Systems Biology, 2333 BE Leiden, The Netherlands.
Children (Basel). 2023 Feb 23;10(3):430. doi: 10.3390/children10030430.
The objective of the current study was to evaluate the potential of 2'-FL and GOS, individually and combined, in beneficially modulating the microbial composition of infant and toddler (12-18 months) feces using the micro-Matrix bioreactor. In addition, the impacts of GOS and 2'-FL, individually and combined, on the outgrowth of fecal bifidobacteria at (sub)species level was investigated using the baby M-SHIME model. For young toddlers, significant increases in the genera , , and , and decreases in , XIVa, and were observed in all supplemented fermentations. In addition, GOS, and combinations of GOS and 2'-FL, increased and decreased , whereas 2'-FL, and combined GOS and 2'-FL, decreased . Alpha diversity increased significantly in infants with GOS and/or 2'-FL, as well as the relative abundances of the genera and with 2'-FL, and with GOS. Combinations of GOS and 2'-FL significantly stimulated , , , and . In all supplemented fermentations, Proteobacteria decreased, with the most profound decreases accomplished by the combination of GOS and 2'-FL. When zooming in on the different (sub)species of , GOS and 2'-FL were shown to be complementary in stimulating breast-fed infant-associated subspecies of in a dose-dependent manner: GOS stimulated subsp. , whereas 2'-FL supported outgrowth of subsp. .
本研究的目的是使用微型基质生物反应器评估2'-岩藻糖基乳糖（2'-FL）和低聚半乳糖（GOS）单独及联合使用对婴幼儿（12 - 18个月）粪便微生物组成进行有益调节的潜力。此外，使用婴儿M-SHIME模型研究了GOS和2'-FL单独及联合使用对粪便双歧杆菌在（亚）种水平上生长的影响。对于幼儿，在所有添加物的发酵过程中，观察到[具体菌属1]、[具体菌属2]、[具体菌属3]菌属显著增加，而[具体菌属4]、[具体菌属5]、XIVa菌属和[具体菌属6]减少。此外，GOS以及GOS与2'-FL的组合增加了[具体菌属7]并减少了[具体菌属8]，而2'-FL以及GOS与2'-FL的组合减少了[具体菌属9]。在食用GOS和/或2'-FL的婴儿中，α多样性显著增加，同时2'-FL使[具体菌属10]和[具体菌属11]的相对丰度增加，GOS使[具体菌属12]的相对丰度增加。GOS和2'-FL的组合显著刺激了[具体菌属13]、[具体菌属14]、[具体菌属15]和[具体菌属16]。在所有添加物的发酵过程中，变形菌门减少，GOS和2'-FL的组合使变形菌门减少最为显著。当关注双歧杆菌的不同（亚）种时，GOS和2'-FL在以剂量依赖方式刺激与母乳喂养婴儿相关的双歧杆菌亚种方面表现出互补性：GOS刺激了[双歧杆菌亚种1]亚种，而2'-FL促进了[双歧杆菌亚种2]亚种的生长。
