通过单细胞转录组学揭示胆管癌肿瘤生态系统的异质性。
Revealing the Heterogeneity of the Tumor Ecosystem of Cholangiocarcinoma through Single-Cell Transcriptomics.
机构信息
Thoracic and GI Malignancies Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA.
Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20814, USA.
出版信息
Cells. 2023 Mar 10;12(6):862. doi: 10.3390/cells12060862.
The prognosis of cholangiocarcinoma remains poor. The heterogeneity of the tumor ecosystem of cholangiocarcinoma plays a critical role in tumorigenesis and therapeutic resistance, thereby affecting the clinical outcome of patients with cholangiocarcinoma. Recent progress in single-cell RNA sequencing (scRNA-seq) has enabled detailed characterization of intratumoral stromal and malignant cells, which has vastly improved our understanding of the heterogeneity of various cell components in the tumor ecosystem of cholangiocarcinoma. It also provides an unprecedented view of the phenotypical and functional diversity in tumor and stromal cells including infiltrating immune cells. This review focuses on examining tumor heterogeneity and the interaction between various cellular components in the tumor ecosystem of cholangiocarcinoma derived from an scRNA-seq dataset, discussing limitations in current studies, and proposing future directions along with potential clinical applications.
胆管癌的预后仍然较差。胆管癌肿瘤生态系统的异质性在肿瘤发生和治疗耐药中起着关键作用，从而影响胆管癌患者的临床结局。单细胞 RNA 测序 (scRNA-seq) 的最新进展使我们能够详细描述肿瘤内基质和恶性细胞，这极大地提高了我们对胆管癌肿瘤生态系统中各种细胞成分异质性的理解。它还为包括浸润免疫细胞在内的肿瘤和基质细胞的表型和功能多样性提供了前所未有的视角。这篇综述重点研究了从 scRNA-seq 数据集得出的胆管癌肿瘤生态系统中的肿瘤异质性和各种细胞成分之间的相互作用，讨论了当前研究的局限性，并提出了未来的方向以及潜在的临床应用。
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