Hume W J
Cell Tissue Kinet. 1986 Mar;19(2):195-203. doi: 10.1111/j.1365-2184.1986.tb00730.x.
Sheet preparations of the stratum granulosum from the epithelium of the ventral surface of mouse tongue permit examination of cell replacement of this maturation compartment of the tissue. The cell transit rate/day is related to the cell desquamation rate and the cell production rate. The latter is approximately 6500-8000 cells/mm2/day, suggesting a 4-5-fold greater turnover compared with mouse dorsal skin epithelium. The use of [3H]IUdR and [3H]TdR at different times of day provides evidence for a reutilization of label from [3H]TdR released during nuclear degradation in the stratum granulosum. Flooding with unlabelled thymidine is not effective in suppressing this reutilization.
从小鼠舌腹面上皮制备颗粒层涂片，可用于研究该组织成熟区室的细胞更替情况。细胞每日转运速率与细胞脱屑速率和细胞生成速率相关。后者约为6500 - 8000个细胞/平方毫米/天，这表明与小鼠背部皮肤上皮相比，细胞更新速度快4 - 5倍。在一天中的不同时间使用[3H]IUdR和[3H]TdR，为颗粒层细胞核降解过程中释放的[3H]TdR的标记再利用提供了证据。用未标记的胸苷进行冲洗并不能有效抑制这种再利用。
