College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China.
Genes (Basel). 2023 Feb 27;14(3):597. doi: 10.3390/genes14030597.
In order to reveal the distribution and expression characteristics of the pulmonary ionocyte-related factors CFTR, ATP6V0D2, and ATP6V1C2 in the lungs of yaks of different ages. Explore the possible regulation of these pulmonary ionocyte-related factors in the yak lung for adaptation to high-altitude hypoxia. The localization and expression of CTFR, ATP6V0D2, and ATP6V1C2 in the lungs of newborn, juvenile, adult, and elderly yaks were studied using immunohistochemistry, quantitative reverse transcription PCR, and Western blotting. Immunohistochemistry showed that CFTR, ATP6V0D2 and ATP6V1C2 were mainly localized in the ciliated cells and club cells of the epithelial mucosal layer of the bronchus and its branches in the lungs. For the qRT-PCR, expression of CFTR, ATP6V0D2 and ATP6V1C2 in the yak lungs varied according to age. For Western blotting, CFTR expression in the newborn group was significantly higher than in the other three groups. ATP6V0D2 expression of the adult group was significantly higher. ATP6V1C2 expression was the highest in the juvenile group ( < 0.05). This study showed that ciliated cells and club cells were related to the pulmonary ionocytes in yaks. CFTR, ATP6V0D2, and ATP6V1C2 were related to adaptations of yak lungs to high altitude hypoxia, through prevention of airway damage.
为了揭示不同年龄牦牛肺中与离子细胞相关的 CFTR、ATP6V0D2 和 ATP6V1C2 因子的分布和表达特征。探讨这些与肺离子细胞相关的因子在牦牛肺中对适应高原低氧的可能调节作用。采用免疫组织化学、定量逆转录 PCR 和 Western blot 法研究 CFTR、ATP6V0D2 和 ATP6V1C2 在新生、幼、成、老年牦牛肺中的定位和表达。免疫组织化学显示，CFTR、ATP6V0D2 和 ATP6V1C2 主要定位于支气管及其分支的上皮黏膜层的纤毛细胞和 club 细胞中。对于 qRT-PCR，CFTR、ATP6V0D2 和 ATP6V1C2 在牦牛肺中的表达随年龄而变化。对于 Western blot，新生组 CFTR 的表达明显高于其他三组。成年组的 ATP6V0D2 表达明显较高。幼龄组的 ATP6V1C2 表达最高（<0.05）。本研究表明，纤毛细胞和 club 细胞与牦牛的肺离子细胞有关。CFTR、ATP6V0D2 和 ATP6V1C2 与牦牛肺对高原低氧的适应有关，通过防止气道损伤。
