自噬和细胞凋亡在等离子体处理盐水、阿霉素和醋酸甲羟孕酮联合作用于 K562 髓系白血病细胞中的作用。

The Role of Autophagy and Apoptosis in the Combined Action of Plasma-Treated Saline, Doxorubicin, and Medroxyprogesterone Acetate on K562 Myeloid Leukaemia Cells.

机构信息

Prokhorov General Physics Institute of the Russian Academy of Sciences, 119991 Moscow, Russia.

Faculty of Biomedicine, Pirogov Russian National Research Medical University, 117997 Moscow, Russia.

出版信息

Int J Mol Sci. 2023 Mar 7;24(6):5100. doi: 10.3390/ijms24065100.

DOI:10.3390/ijms24065100

PMID:36982174

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10049101/

Abstract

The anti-cancer properties of plasma-treated solutions (PTS) and their interaction with drugs are one of the most popular topics in modern plasma medicine. Our research involved comparing the effects of four physiological saline solutions (0.9% NaCl, Ringer's solution, Hank's Balanced Salt Solution, Hank's Balanced Salt Solution with amino acids added in concentrations observed in the human blood) treated with cold atmospheric plasma and studying the combined cytotoxic effect of PTS with doxorubicin and medroxyprogesterone acetate (MPA). Analysis of the effect of the studied agents on the formation of radicals in the incubation medium, the vitality of K562 myeloid leukaemia cells, and the processes of autophagy and apoptosis in them revealed two key findings. The first is that when using PTS and doxorubicin-containing PTS, autophagy is the predominant process in cancer cells. The second is that combining PTS with MPA enhances apoptotic processes. It was hypothesised that while autophagy is stimulated by the accumulation of reactive oxygen species in the cell, apoptosis is stimulated through specific cell progesterone receptors.

摘要

等离子体处理溶液（PTS）的抗癌特性及其与药物的相互作用是现代等离子体医学中最热门的话题之一。我们的研究涉及比较四种生理盐水溶液（0.9% NaCl、林格氏溶液、Hank's 平衡盐溶液、添加了人类血液中浓度观察到的氨基酸的 Hank's 平衡盐溶液）经冷大气压等离子体处理后的效果，并研究 PTS 与多柔比星和醋酸甲地孕酮（MPA）联合的细胞毒性作用。分析研究药物对孵育介质中自由基形成、K562 髓性白血病细胞活力以及其中自噬和细胞凋亡过程的影响，揭示了两个关键发现。第一个发现是，当使用 PTS 和含有多柔比星的 PTS 时，自噬是癌细胞中的主要过程。第二个发现是，将 PTS 与 MPA 结合可增强细胞凋亡过程。据推测，自噬是通过细胞内活性氧物质的积累而被刺激的，而凋亡则是通过特定的细胞孕激素受体被刺激的。