Francoual J, Lindenbaum A, Benattar C, Dehan M, Cohen H, Leluc R
Clin Chem. 1986 May;32(5):877-8.
To detect the presence of meconium and blood, we determined coproporphyrin and hemoglobin in 78 amniotic fluid samples, classified according to their visual aspect as clear (n = 45) or meconium-stained (n = 33). Among the 33 samples visually judged as meconial, a comparison of the results of their biochemical analysis with the mean values for clear amniotic fluids showed that only five of the 33 were contaminated solely with meconium. Seven others were exclusively bloody, and 19 were meconial and bloody. In two, the contaminant was neither meconium nor blood. Evidently, when the fluid is brownish, simple visual examination does not suffice for distinguishing meconium from old blood or other pigments. We propose a simple, rapid, and reliable method for simultaneously measuring contamination with meconium or blood.
为检测胎粪和血液的存在，我们测定了78份羊水样本中的粪卟啉和血红蛋白，这些样本根据外观分为清亮（n = 45）或胎粪污染（n = 33）两类。在33份肉眼判断为胎粪污染的样本中，将其生化分析结果与清亮羊水的平均值进行比较，结果显示33份样本中只有5份仅被胎粪污染。另外7份样本仅为血性，19份样本既被胎粪污染又有血性。有两份样本的污染物既不是胎粪也不是血液。显然，当羊水呈褐色时，单纯的肉眼检查不足以区分胎粪与陈旧血液或其他色素。我们提出一种简单、快速且可靠的方法，用于同时测定胎粪或血液污染情况。
