Westerkowsky Elisa Katja, Soares de Almeida Adriana Marisa, Selle Michael, Harms Oliver, Bundkirchen Katrin, Neunaber Claudia, Noack Sandra
Hannover Medical School, Department of Trauma Surgery, Carl-Neuberg-Straße 1, 30625 Hannover, Germany.
Clinic for Small Animal Medicine, University of Veterinary Medicine Hannover, Bünteweg 9, 30559 Hannover, Germany.
Life (Basel). 2023 Mar 6;13(3):718. doi: 10.3390/life13030718.
For research and clinical use of stem cells, a suitable animal model is necessary. Hence, the aim of this study was to compare human-bone-marrow-derived mesenchymal stem cells (hBMSCs) with those from sheep (oBMSCs) and pigs (pBMSCs). The cells from these three species were examined for their self-renewal potential; proliferation potential; adhesion and migration capacity; adipogenic, osteogenic and chondrogenic differentiation potential; and cell morphology. There was no significant difference between hBMSCs and pBMSCs in terms of self-renewal potential or growth potential. The oBMSCs exhibited a significantly higher doubling time than hBMSCs from passage 7. The migration assay showed significant differences between hBMSCs and pBMSCs and oBMSCs-up to 30 min, hBMSCs were faster than both types and after 60 min faster than pBMSCs. In the adhesion assay, hBMSCs were significantly better than oBMSCs and pBMSCs. When differentiating in the direction of osteogenesis, oBMSCs and pBMSCs have shown a clearer osteogenic potential. In all three species, adipogenesis could only be evaluated qualitatively. The chondrogenic differentiation was successful in hBMSCs and pBMSCs in contrast to oBMSCs. It is also important to note that the cell size of pBMSCs was significantly smaller compared to hBMSCs. Finally, it can be concluded that further comparative studies are needed to draw a clear comparison between hBMSCs and pBMSCs/oBMSCs.
对于干细胞的研究和临床应用而言，合适的动物模型是必要的。因此，本研究的目的是比较人骨髓间充质干细胞（hBMSCs）与绵羊（oBMSCs）和猪（pBMSCs）的间充质干细胞。对这三个物种的细胞进行了自我更新潜力、增殖潜力、黏附与迁移能力、脂肪生成、成骨和成软骨分化潜力以及细胞形态的检测。hBMSCs和pBMSCs在自我更新潜力或生长潜力方面没有显著差异。从第7代开始，oBMSCs的倍增时间显著高于hBMSCs。迁移试验显示hBMSCs与pBMSCs和oBMSCs之间存在显著差异——在30分钟内，hBMSCs比这两种细胞都快，60分钟后比pBMSCs快。在黏附试验中，hBMSCs明显优于oBMSCs和pBMSCs。在向成骨方向分化时，oBMSCs和pBMSCs显示出更明显的成骨潜力。在所有三个物种中，脂肪生成只能进行定性评估。与oBMSCs不同，hBMSCs和pBMSCs的成软骨分化是成功的。还需要注意的是，与hBMSCs相比，pBMSCs的细胞大小显著更小。最后，可以得出结论，需要进一步的比较研究才能在hBMSCs与pBMSCs/oBMSCs之间进行明确的比较。
