Salustri Alessandro, De Maio Flavio, Palmieri Valentina, Santarelli Giulia, Palucci Ivana, Mercedes Bianco Delia, Marchionni Federica, Bellesi Silvia, Ciasca Gabriele, Perini Giordano, Sanguinetti Maurizio, Sali Michela, Papi Massimiliano, De Spirito Marco, Delogu Giovanni
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie-Sezione di Microbiologia, Università Cattolica del Sacro Cuore, 00168 Rome, Italy.
Dipartimento di Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario "A. Gemelli", IRCCS, 00168 Rome, Italy.
Microorganisms. 2023 Feb 22;11(3):554. doi: 10.3390/microorganisms11030554.
Graphene Oxide has been proposed as a potential adjuvant to develop improved anti-TB treatment, thanks to its activity in entrapping mycobacteria in the extracellular compartment limiting their entry in macrophages. Indeed, when administered together with linezolid, Graphene Oxide significantly enhanced bacterial killing due to the increased production of Reactive Oxygen Species. In this work, we evaluated Graphene Oxide toxicity and its anti-mycobacterial activity on human peripheral blood mononuclear cells. Our data show that Graphene Oxide, different to what is observed in macrophages, does not support the clearance of in human immune primary cells, probably due to the toxic effects of the nano-material on monocytes and CD4+ lymphocytes, which we measured by cytometry. These findings highlight the need to test GO and other carbon-based nanomaterials in relevant in vitro models to assess the cytotoxic activity while measuring antimicrobial potential.
氧化石墨烯已被提议作为一种潜在的佐剂，用于开发改进的抗结核治疗方法，这得益于其在细胞外隔室中捕获分枝杆菌，限制其进入巨噬细胞的活性。事实上，当与利奈唑胺一起给药时，氧化石墨烯由于活性氧的产生增加而显著增强了细菌杀伤作用。在这项工作中，我们评估了氧化石墨烯对人外周血单个核细胞的毒性及其抗分枝杆菌活性。我们的数据表明，与在巨噬细胞中观察到的情况不同，氧化石墨烯在人免疫原代细胞中不支持清除作用，这可能是由于纳米材料对单核细胞和CD4 +淋巴细胞的毒性作用，我们通过细胞计数法进行了测量。这些发现凸显了在相关体外模型中测试氧化石墨烯和其他碳基纳米材料的必要性，以便在测量抗菌潜力的同时评估细胞毒性活性。
