Petrzik Karel, Vacek Josef, Kmoch Martin, Binderová Denisa, Brázdová Sára, Lenz Ondřej, Ševčík Rudolf
Institute of Molecular Biology, Biology Centre of the Czech Academy of Sciences, Branišovská 1160/31, 370 05 České Budějovice, Czech Republic.
Department of Growing Technologies, Potato Research Institute Havlíčkův Brod, Dobrovského 2366, 580 01 Havlíčkův Brod, Czech Republic.
Microorganisms. 2023 Feb 28;11(3):620. doi: 10.3390/microorganisms11030620.
The pectinolytic bacterium is an important pathogen found in potatoes. We conducted laboratory and field experiments mimicking severe and mild spp. infection and investigated the application of a mixture of two lytic bacteriophages before and after bacterial infection to protect the plants. Application of the phage solution to tuber disks and wounded tubers did not completely eliminate the infection but reduced the development of soft rot symptoms by 59.5-91.4%, depending on the phage concentration. In the field trial, plants treated with bacteriophages after severe infection had 5-33% greater leaf cover and 4-16% greater tuber yield compared to untreated plants. When simulating a mild infection, leaf cover was 11-42% greater, and tuber yield was 25-31% greater compared to untreated plants. We conclude that the phage mixture has the potential to protect potatoes ecologically from .
果胶分解细菌是在马铃薯中发现的一种重要病原体。我们进行了模拟严重和轻度感染的实验室和田间试验，并研究了在细菌感染前后应用两种裂解性噬菌体的混合物来保护植株。将噬菌体溶液应用于块茎切片和受伤的块茎上并不能完全消除感染，但根据噬菌体浓度的不同，可将软腐症状的发展减少59.5%至91.4%。在田间试验中，与未处理的植株相比，在严重感染后用噬菌体处理的植株叶面积覆盖率高5%至33%，块茎产量高4%至16%。当模拟轻度感染时，与未处理的植株相比，叶面积覆盖率高11%至42%，块茎产量高25%至31%。我们得出结论，噬菌体混合物有潜力在生态上保护马铃薯免受……
