Zamorano Diego, Ivulic Dinka, Viver Tomeu, Morales Felipe, López-Kostner Francisco, Vidal Roberto M
Colorrectal Unit, Clínica Las Condes, Santiago 13114, Chile.
Programa de Microbiología y Micología, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380453, Chile.
Microorganisms. 2023 Mar 7;11(3):680. doi: 10.3390/microorganisms11030680.
Anastomotic leakage (AL) is a major cause of morbidity and mortality after colorectal surgery, but the mechanism behind this complication is still not fully understood. Despite the advances in surgical techniques and perioperative care, the complication rates have remained steady. Recently, it has been suggested that colon microbiota may be involved in the development of complications after colorectal surgery. The aim of this study was to evaluate the association of gut microbiota in the development of colorectal AL and their possible virulence strategies to better understand the phenomenon. Using 16S rRNA sequencing of samples collected on the day of surgery and the sixth day following surgery, we analyzed the changes in tissue-associated microbiota at anastomotic sites created in a model of rats with ischemic colon resection. We discovered a trend for lower microbial diversity in the AL group compared to non-leak anastomosis (NLA). There were no differences in relative abundance in the different types of microbial respiration between these groups and the high abundance of the facultative anaerobic is a marker species that stands out as a distinctive feature.
吻合口漏（AL）是结直肠手术后发病和死亡的主要原因，但这种并发症背后的机制仍未完全明确。尽管手术技术和围手术期护理有所进步，但并发症发生率仍保持稳定。最近，有人提出结肠微生物群可能与结直肠手术后并发症的发生有关。本研究的目的是评估肠道微生物群在结直肠AL发生中的关联及其可能的致病策略，以更好地理解这一现象。通过对手术当天和术后第六天采集的样本进行16S rRNA测序，我们分析了在缺血性结肠切除大鼠模型中创建的吻合口部位组织相关微生物群的变化。我们发现，与无漏吻合（NLA）相比，AL组的微生物多样性有降低趋势。这些组之间不同类型微生物呼吸的相对丰度没有差异，兼性厌氧菌的高丰度是一个突出的标志性物种。
