Tang Xiaoyu, Zhang Ruiyu, Gao Long, Lv Xiaocheng, Sun Yuan, Ma Jingyun
Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, China.
Microorganisms. 2023 Mar 8;11(3):688. doi: 10.3390/microorganisms11030688.
Seneca Valley virus (SVV), a member of the Picornaviridae family, is an oncolytic RNA virus that can cause idiopathic vesicular disease and increase mortality in newborn piglets. Although research on the pathogenic characteristics, epidemiology, pathogenic mechanism, and clinical diagnosis of SVA has increased due to its emergence and prevalence, the interaction between SVA and its host lncRNA has not been fully studied. This study used qualcomm sequencing to analyze differentially expressed lncRNAs and found that during SVA infection, lncRNA 8244 was significantly down-regulated in both PK-15 cells and piglets. Further analysis through quantitative real-time PCR and dual luciferase experiments demonstrated that lncRNA8244 could compete with ssc-miR-320 to regulate the expression of CCR7. The lncRNA824-ssc-miR-320-CCR7 axis activated the TLR-mediated signaling pathway, which recognized viral molecules and induced the expression of IFN-β. These findings provide new insight into the interaction between lncRNA and SVA infection, which could lead to a better understanding of SVA pathogenesis and contribute to the prevention and control of SVA disease.
塞内卡山谷病毒（SVV）是小核糖核酸病毒科的成员，是一种溶瘤性RNA病毒，可引起特发性水疱病并增加新生仔猪的死亡率。尽管由于SVA的出现和流行，对其致病特征、流行病学、致病机制和临床诊断的研究有所增加，但SVA与其宿主长链非编码RNA（lncRNA）之间的相互作用尚未得到充分研究。本研究采用高通量测序分析差异表达的lncRNA，发现在SVA感染期间，lncRNA 8244在PK-15细胞和仔猪中均显著下调。通过定量实时PCR和双荧光素酶实验进一步分析表明，lncRNA8244可以与ssc-miR-320竞争以调节CCR7的表达。lncRNA824-ssc-miR-320-CCR7轴激活了TLR介导的信号通路，该通路识别病毒分子并诱导IFN-β的表达。这些发现为lncRNA与SVA感染之间的相互作用提供了新的见解，这可能有助于更好地理解SVA的发病机制，并有助于SVA疾病的预防和控制。
